Human ATCAY ELISA Kit

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  • Alternative name

    Human ataxia cayman type protein ELISA Kit;Human BNIP-2-homology ELISA Kit;Human BNIP-H ELISA Kit;Human KIAA1872 ELISA Kit;Human CLAC ELISA Kit;Human ATCAY, caytaxin ELISA Kit;Human caytaxin ELISA Kit;Human BNIP-2-homolgy ELISA Kit;Human Cayman ataxia ELISA Kit;Human ataxia cerebellar Cayman type ELISA Kit;

  • Catalog
    E007133
  • species
    Human
  • GeneATCAY
  • Standard CurveHuman ATCAY ELISA Kit
  • Other Species Mouse Atcay ELISA KitMonkey ATCAY ELISA KitRat Atcay ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of ATCAY. No significant cross-reactivity or interference between ATCAY and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ATCAY and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman ATCAY ELISA Kit allows for the in vitro quantitative determination of ATCAY , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyNeurobiology
  • Product Description
    specifical
    Intended Uses: This ATCAY ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ATCAY. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: ATCAY ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ATCAY antibody and an ATCAY-HRP conjugate. The assay sample and buffer are incubated together with ATCAY-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ATCAY concentration since ATCAY from samples and ATCAY-HRP conjugate compete for the anti-ATCAY antibody binding site. Since the number of sites is limited, as more sites are occupied by ATCAY from the sample, fewer sites are left to bind ATCAY-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ATCAY concentration in each sample is interpolated from this standard curve.
  • Human Caytaxin Protein information
  • Uniprot ID ATCAY_HUMAN
  • Uniprot AC Q86WG3; Q8NAQ2; Q8TAQ3; Q96HC6; Q96JF5;
  • UniGene Hs.418055;
  • GeneID 85300
  • KEGG hsa:85300;
  • Human Caytaxin Protein SEQUENCE
  • SEQUENCE 371 AA; 42120 MW; C09174CE3AD77764 CRC64;

    MGTTEATLRM ENVDVKEEWQ DEDLPRPLPE ETGVELLGSP VEDTSSPPNT

    LNFNGAHRKR KTLVAPEINI SLDQSEGSLL SDDFLDTPDD LDINVDDIET

    PDETDSLEFL GNGNELEWED DTPVATAKNM PGDSADLFGD GTTEDGSAAN

    GRLWRTVIIG EQEHRIDLHM IRPYMKVVTH GGYYGEGLNA IIVFAACFLP

    DSSLPDYHYI MENLFLYVIS SLELLVAEDY MIVYLNGATP RRRMPGIGWL

    KKCYQMIDRR LRKNLKSLII VHPSWFIRTV LAISRPFISV KFINKIQYVH

    SLEDLEQLIP MEHVQIPDCV LQYEEERLKA RRESARPQPE FVLPRSEEKP

    EVAPVENRSA LVSEDQETSM S

  • UCSC uc002lyy.5; human. [Q86WG3-1];




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