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Mouse Tpp1 ELISA Kit

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  • Alternative name

    Mouse TPP-1 ELISA Kit;Mouse lysosomal pepstatin-insensitive protease ELISA Kit;Mouse LPIC ELISA Kit;Mouse tripeptidyl aminopeptidase ELISA Kit;Mouse Tripeptidyl-peptidase I ELISA Kit;Mouse TPP-I ELISA Kit;Mouse Cln2 ELISA Kit;Mouse tripeptidyl peptidase I ELISA Kit;Mouse tripeptidyl-peptidase 1 ELISA Kit;Mouse ceroid-lipofuscinosis, neuronal 2 ELISA Kit;
  • Catalog
    E071216
  • species
    Mouse
  • GeneTpp1
  • Standard CurveMouse Tpp1 ELISA Kit
  • Other Species Human TPP1 ELISA KitBovine TPP1 ELISA KitCanine TPP1 ELISA KitMonkey TPP1 ELISA KitRat Tpp1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Tripeptidyl Peptidase I (TPP1). No significant cross-reactivity or interference between Tripeptidyl Peptidase I (TPP1) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.055ng/mL.
  • Intended UseMouse Tpp1 ELISA Kit allows for the in vitro quantitative determination of Tpp1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tripeptidyl Peptidase I (TPP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tripeptidyl Peptidase I (TPP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tripeptidyl Peptidase I (TPP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tripeptidyl Peptidase I (TPP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Mouse Tripeptidyl-peptidase 1 Protein information
  • Uniprot ID TPP1_MOUSE
  • Uniprot AC O89023; Q543Q8; Q9QUS7;
  • UniGene Mm.20837;
  • GeneID 12751
  • KEGG mmu:12751;
  • Mouse Tripeptidyl-peptidase 1 Protein SEQUENCE

  • SEQUENCE 562 AA; 61342 MW; 0AF8163EA1A66396 CRC64;

    MGLQARLLGL LALVIAGKCT YNPEPDQRWM LPPGWVSLGR VDPEEELSLT

    FALKQRNLER LSELVQAVSD PSSPQYGKYL TLEDVAELVQ PSPLTLLTVQ

    KWLSAAGARN CDSVTTQDFL TCWLSVRQAE LLLPGAEFHR YVGGPTKTHV

    IRSPHPYQLP QALAPHVDFV GGLHRFPPSS PRQRPEPQQV GTVSLHLGVT

    PSVLRQRYNL TAKDVGSGTT NNSQACAQFL EQYFHNSDLT EFMRLFGGSF

    THQASVAKVV GKQGRGRAGI EASLDVEYLM SAGANISTWV YSSPGRHEAQ

    EPFLQWLLLL SNESSLPHVH TVSYGDDEDS LSSIYIQRVN TEFMKAAARG

    LTLLFASGDT GAGCWSVSGR HKFRPSFPAS SPYVTTVGGT SFKNPFLITD

    EVVDYISGGG FSNVFPRPPY QEEAVAQFLK SSSHLPPSSY FNASGRAYPD

    VAALSDGYWV VSNMVPIPWV SGTSASTPVF GGILSLINEH RILNGRPPLG

    FLNPRLYQQH GTGLFDVTHG CHESCLNEEV EGQGFCSGPG WDPVTGWGTP

    NFPALLKTLL NP

  • UCSC uc009izg.1; mouse.;


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