Human CHAC1 ELISA Kit

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  • Alternative name

    Human gamma-GCG 1 ELISA Kit;Human blocks Notch protein ELISA Kit;Human botch ELISA Kit;Human Cation transport regulator-like protein 1 ELISA Kit;Human ChaC glutathione specific gamma-glutamylcyclotransferase 1 ELISA Kit;Human glutathione-specific gamma-glutamylcyclotransferase 1 ELISA Kit;Human ChaC, cation transport regulator homolog 1 ELISA Kit;Human ChaC, cation transport regulator-like 1 ELISA Kit;Human gamma-GCT acting on glutathione homolog 1 ELISA Kit;

  • Catalog
    E007102
  • species
    Human
  • GeneCHAC1
  • Standard CurveHuman CHAC1 ELISA Kit
  • Other Species Mouse Chac1 ELISA KitRat Chac1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of CHAC1. No significant cross-reactivity or interference between CHAC1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CHAC1 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 pg/mL.
  • Intended UseHuman CHAC1 ELISA Kit allows for the in vitro quantitative determination of CHAC1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyCell Biology
  • Product Description
    specifical
    Intended Uses||This CHAC1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CHAC1. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: CHAC1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CHAC1 antibody and an CHAC1-HRP conjugate. The assay sample and buffer are incubated together with CHAC1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CHAC1 concentration since CHAC1 from samples and CHAC1-HRP conjugate compete for the anti-CHAC1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CHAC1 from the sample, fewer sites are left to bind CHAC1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D) to the concentration of standards. The CHAC1 concentration in each sample is interpolated from this standard curve.
  • Human Glutathione-specific gamma-glutamylcyclotransferase 1 Protein information
  • Uniprot ID CHAC1_HUMAN
  • Uniprot AC Q9BUX1; Q0VIA0;
  • UniGene Hs.155569;
  • GeneID 79094
  • KEGG hsa:79094;
  • Human Glutathione-specific gamma-glutamylcyclotransferase 1 Protein SEQUENCE
  • SEQUENCE 264 AA; 28747 MW; 746047C1AD03D375 CRC64;

    MGGAQLELPS GARPGVCVRR SFRAHAGDQP RRPPGPIPVP GTMKQESAAP

    NTPPTSQSPT PSAQFPRNDG DPQALWIFGY GSLVWRPDFA YSDSRVGFVR

    GYSRRFWQGD TFHRGSDKMP GRVVTLLEDH EGCTWGVAYQ VQGEQVSKAL

    KYLNVREAVL GGYDTKEVTF YPQDAPDQPL KALAYVATPQ NPGYLGPAPE

    EAIATQILAC RGFSGHNLEY LLRLADFMQL CGPQAQDEHL AAIVDAVGTM

    LPCFCPTEQA LALV

  • UCSC uc001znh.2; human. [Q9BUX1-1];




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