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Mouse Mafb ELISA Kit

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  • Alternative name

    Mouse maf-B ELISA Kit;Mouse Kreisler ELISA Kit;Mouse segmentation protein Kr ELISA Kit;Mouse transcription factor Maf-1 ELISA Kit;Mouse V-maf musculoaponeurotic fibrosarcoma oncogene homolog B ELISA Kit;Mouse Krml ELISA Kit;Mouse Maf1 ELISA Kit;Mouse Krml1 ELISA Kit;Mouse kr ELISA Kit;Mouse v-maf musculoaponeurotic fibrosarcoma oncogene family, protein B (avian) ELISA Kit;Mouse transcription factor MafB ELISA Kit;Mouse Kreisler (maf-related) leucine zipper protein ELISA Kit;
  • Catalog
    E070598
  • species
    Mouse
  • GeneMafb
  • Standard CurveMouse Mafb ELISA Kit
  • Other Species Human MAFB ELISA KitChicken MAFB ELISA KitMonkey MAFB ELISA KitRat Mafb ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of MAFB. No significant cross-reactivity or interference between MAFB and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between MAFB and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseMouse Mafb ELISA Kit allows for the in vitro quantitative determination of Mafb , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyEpigenetics and Nuclear Signaling
  • Product Description
    specifical    Intended Uses: This MAFB ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse MAFB. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: MAFB ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-MAFB antibody and an MAFB-HRP conjugate. The assay sample and buffer are incubated together with MAFB-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MAFB concentration since MAFB from samples and MAFB-HRP conjugate compete for the anti-MAFB antibody binding site. Since the number of sites is limited, as more sites are occupied by MAFB from the sample, fewer sites are left to bind MAFB-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MAFB concentration in each sample is interpolated from this standard curve.
  • Mouse Transcription factor MafB Protein information
  • Uniprot ID MAFB_MOUSE
  • Uniprot AC P54841; Q3U3C8; Q3UPT9;
  • UniGene Mm.330745;
  • GeneID 16658
  • KEGG mmu:16658;
  • Mouse Transcription factor MafB Protein SEQUENCE

  • SEQUENCE 323 AA; 35809 MW; D77AE07ABD9C2AD2 CRC64;

    MAAELSMGQE LPTSPLAMEY VNDFDLLKFD VKKEPLGRAE RPGRPCTRLQ

    PAGSVSSTPL STPCSSVPSS PSFSPTEPKT HLEDLYWMAS NYQQMNPEAL

    NLTPEDAVEA LIGSHPVPQP LQSFDGFRSA HHHHHHHHPH PHHGYPGAGV

    THDDLGQHAH PHHHHHHQAS PPPSSAASPA QQLPTSHPGP GPHATAAATA

    AGGNGSVEDR FSDDQLVSMS VRELNRHLRG FTKDEVIRLK QKRRTLKNRG

    YAQSCRYKRV QQKHHLENEK TQLIQQVEQL KQEVSRLARE RDAYKVKCEK

    LANSGFREAG STSDSPSSPE FFL

  • UCSC uc008nqw.2; mouse.;


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