Mouse TSST-1 ELISA Kit

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  • Alternative name

    Toxic shock syndrome toxin-1 ELISA KIT; tst ELISA KIT; TSST-1 ELISA KIT

  • Catalog
    E070464
  • species
    Mouse
  • GeneTSST-1
  • Standard CurveMouse TSST-1 ELISA Kit
  • Other Species Human TSST-1 ELISA KitHuman TSST 1 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseMouse TSST-1 ELISA Kit allows for the in vitro quantitative determination of TSST-1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    For Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate Intended Uses: This TSST-1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse TSST-1. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: TSST-1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for TSST-1. Standards or samples are then added to the microtiter plate wells and TSST-1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of TSST-1 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for TSST-1 are added to each well to "sandwich" the TSST-1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain TSST-1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TSST-1 concentration in each sample is interpolated from this standard curve.


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