Human CTSG ELISA Kit

Alternative name
Human CG ELISA Kit;Human CATG ELISA Kit;Human cathepsin G ELISA Kit;
Catalog
E007043
species
Human
GeneCTSG
SpecificityThe microplate is coated with Cathepsin G. The antigen preparation is highly purified by affinity chromatography. The Anti-Cathepsin G test is specific only for autoantibodies directed against anti-Cathepsin G. No cross-reactivities to the other ANCA antigens have been observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe lower detection limit for the Anti-Cathepsin G test was determined at 0.5 U/ml.
Intended UseHuman CTSG ELISA Kit allows for the in vitro quantitative determination of CTSG , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyAutoimmune; Immunology; Research Assays; Assay Categories
Product Description
specificalPrinciple of the assay: Highly purified Cathepsin G is bound to microwells. Antibodies against this antigen, if present in diluted serum, bind to the respective antigen. Washing of the microwells removes unspecific serum and plasma components. Horseradish peroxidase (HRP) conjugated anti-human IgG immunologically detects the bound antibodies forming a conjugate/anti-body/antigen complex. Washing of the microwells removes unbound conjugate. An enzyme substrate in the presence of bound conjugate hydrolyzes to form a blue colour. The addition of an acid stops the reaction forming a yellow endproduct. The intensity of this yellow colour is measured photometrically at 450 nm. The amount of colour is directly proportional to the concentration of IgG antibodies present in the original sample.
Human Cathepsin G Protein information
Uniprot ID CATG_HUMAN
Uniprot AC P08311; Q6IBJ6; Q9UCA5; Q9UCU6;
UniGene Hs.421724;
GeneID 1511
KEGG hsa:1511;
Human Cathepsin G Protein SEQUENCE
SEQUENCE 255 AA; 28837 MW; 6228E741E6A43889 CRC64;
MQPLLLLLAF LLPTGAEAGE IIGGRESRPH SRPYMAYLQI QSPAGQSRCG
GFLVREDFVL TAAHCWGSNI NVTLGAHNIQ RRENTQQHIT ARRAIRHPQY
NQRTIQNDIM LLQLSRRVRR NRNVNPVALP RAQEGLRPGT LCTVAGWGRV
SMRRGTDTLR EVQLRVQRDR QCLRIFGSYD PRRQICVGDR RERKAAFKGD
SGGPLLCNNV AHGIVSYGKS SGVPPEVFTR VSSFLPWIRT TMRSFKLLDQ
METPL
UCSC uc001wpq.4; human.;

Category

Human CTSG ELISA Kit

Human CG ELISA Kit;Human CATG ELISA Kit;Human cathepsin G ELISA Kit;