Mouse Tnfsf14 ELISA Kit

Alternative name
Mouse LIGHT ELISA Kit;Mouse HVEM-L ELISA Kit;Mouse HVEML ELISA Kit;Mouse LTg ELISA Kit;Mouse Ly113 ELISA Kit;Mouse Tnlg1d ELISA Kit;Mouse tumor necrosis factor (ligand) superfamily, member 14 ELISA Kit;Mouse tumor necrosis factor ligand superfamily member 14 ELISA Kit;Mouse tumor necrosis factor ligand 1d ELISA Kit;
Catalog
E070335
species
Mouse
GeneTnfsf14
SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse TNFSF14. No significant cross-reactivity or interference between Mouse TNFSF14 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse TNFSF14 and all the analogues, therefore, cross reaction may still exist.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity9.375pg/mL
Intended UseMouse Tnfsf14 ELISA Kit allows for the in vitro quantitative determination of Tnfsf14 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalDescription: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of TNFSF14 in Mouse serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse TNFSF14. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse TNFSF14 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse TNFSF14, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse TNFSF14. You can calculate the concentration of Mouse TNFSF14 in the samples by comparing the OD of the samples to the standard curve.
Mouse Tumor necrosis factor ligand superfamily member 14, soluble form Protein information
Uniprot ID TNF14_MOUSE
Uniprot AC Q9QYH9; Q059Y9;
UniGene Mm.483369;
GeneID 50930
KEGG mmu:50930;
Mouse Tumor necrosis factor ligand superfamily member 14, soluble form Protein SEQUENCE
SEQUENCE 239 AA; 26338 MW; 217874AC71AD6BE3 CRC64;
MESVVQPSVF VVDGQTDIPF RRLEQNHRRR RCGTVQVSLA LVLLLGAGLA
TQGWFLLRLH QRLGDIVAHL PDGGKGSWEK LIQDQRSHQA NPAAHLTGAN
ASLIGIGGPL LWETRLGLAF LRGLTYHDGA LVTMEPGYYY VYSKVQLSGV
GCPQGLANGL PITHGLYKRT SRYPKELELL VSRRSPCGRA NSSRVWWDSS
FLGGVVHLEA GEEVVVRVPG NRLVRPRDGT RSYFGAFMV
UCSC uc008def.2; mouse.;

Mouse Tnfsf14 ELISA Kit

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