Human CTSE ELISA Kit

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  • Alternative name

    Human CATE ELISA Kit;Human cathepsin E ELISA Kit;Human erythrocyte membrane aspartic proteinase ELISA Kit;Human slow-moving proteinase ELISA Kit;

  • Catalog
    E007033
  • species
    Human
  • GeneCTSE
  • Other Species Mouse Ctse ELISA KitGuinea Pig CTSE ELISA KitRabbit CTSE ELISA KitRat Ctse ELISA Kit
  • SpecificityThe Human Cathepsin E ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Human Cathepsin E proteins within the range of 156 pg/ml - 10000 pg/ml.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity10 pg/ml.
  • Intended UseHuman CTSE ELISA Kit allows for the in vitro quantitative determination of CTSE , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay||The Human Cathepsin E ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Cathepsin E in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Human Cathepsin E coated on a 96-well plate. Standards and samples are pipetted into the wells and Cathepsin E present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Cathepsin E antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Cathepsin E bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Background/Introduction: Cathepsin E is a protein that in humans is encoded by the CTSE gene. It is mapped to 1q32.1. The protein encoded by this gene is a gastric aspartyl protease that functions as a disulfide-linked homodimer. This protease, which is a member of the peptidase C1 family, has a specificity similar to that of pepsin A and cathepsin D. It is an intracellular proteinase that does not appear to be involved in the digestion of dietary protein and is found in highest concentration in the surface of epithelial mucus-producing cells of the stomach. Cathepsin E is the first aspartic proteinase expressed in the fetal stomach and is found in more than half of gastric cancers. It appears, therefore, to be an oncofetal antigen, and it also has an important role in immune responses.
  • Human Cathepsin E form II Protein information
  • Uniprot ID CATE_HUMAN
  • Uniprot AC P14091; Q5TZ01; Q5TZ02; Q9NY58; Q9UCE3; Q9UCE4;
  • UniGene Hs.644082;
  • GeneID 1510
  • KEGG hsa:1510;
  • Human Cathepsin E form II Protein SEQUENCE
  • SEQUENCE 401 AA; 43312 MW; 46A2BA35266032CB CRC64;

    MKTLLLLLLV LLELGEAQGS LHRVPLRRHP SLKKKLRARS QLSEFWKSHN

    LDMIQFTESC SMDQSAKEPL INYLDMEYFG TISIGSPPQN FTVIFDTGSS

    NLWVPSVYCT SPACKTHSRF QPSQSSTYSQ PGQSFSIQYG TGSLSGIIGA

    DQVSAFATQV EGLTVVGQQF GESVTEPGQT FVDAEFDGIL GLGYPSLAVG

    GVTPVFDNMM AQNLVDLPMF SVYMSSNPEG GAGSELIFGG YDHSHFSGSL

    NWVPVTKQAY WQIALDNIQV GGTVMFCSEG CQAIVDTGTS LITGPSDKIK

    QLQNAIGAAP VDGEYAVECA NLNVMPDVTF TINGVPYTLS PTAYTLLDFV

    DGMQFCSSGF QGLDIHPPAG PLWILGDVFI RQFYSVFDRG NNRVGLAPAV

    P

  • UCSC uc001hdu.3; human. [P14091-3];




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