Human CTSD ELISA Kit

Alternative name
Human CPSD ELISA Kit;Human CLN10 ELISA Kit;Human HEL-S-130P ELISA Kit;Human cathepsin D ELISA Kit;Human ceroid-lipofuscinosis, neuronal 10 ELISA Kit;Human epididymis secretory sperm binding protein Li 130P ELISA Kit;Human lysosomal aspartyl peptidase ELISA Kit;Human lysosomal aspartyl protease ELISA Kit;
Catalog
E007020
species
Human
GeneCTSD
Standard Curve
Other Species Mouse Ctsd ELISA Kit Bovine CTSD ELISA Kit Canine CTSD ELISA Kit Chicken CTSD ELISA Kit Porcine CTSD ELISA Kit Rat Ctsd ELISA Kit Sheep CTSD ELISA Kit
SpecificityNatural and recombinant human Cathepsin D
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 10pg/ml
Intended UseHuman CTSD ELISA Kit allows for the in vitro quantitative determination of CTSD , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: human Cathepsin D ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Cathepsin D has been precoated onto 96-well plates. Standards(NSO, L21-L412) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Cathepsin D is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Cathepsin D amount of sample captured in plate. Background: Cathepsin D is a protein that in humans is encoded by the CTSD gene. This proteinase is a member of the peptidase C1 family, having a specificity similar to but narrower than that of pepsin A. It is mapped to 11p15.5. The cDNA encodes a 412-amino acid protein with 20 and 44 amino acids in a pre- and prosegment, respectively. Cathepsin D is one of the lysosomal proteinases. It is ubiquitously expressed and is involved in proteolytic degradation, cell invasion, and apoptosis. Mutations in this gene are involved in the pathogenesis of several diseases, including breast cancer and possibly Alzheimer disease and it has been considered as a breast cancer tumor marker.
Human Cathepsin D heavy chain Protein information
Uniprot ID CATD_HUMAN
Uniprot AC P07339; Q6IB57;
UniGene Hs.654447;
GeneID 1509
KEGG hsa:1509;
Human Cathepsin D heavy chain Protein SEQUENCE
SEQUENCE 412 AA; 44552 MW; 903FB8412E0CF0B0 CRC64;
MQPSSLLPLA LCLLAAPASA LVRIPLHKFT SIRRTMSEVG GSVEDLIAKG
PVSKYSQAVP AVTEGPIPEV LKNYMDAQYY GEIGIGTPPQ CFTVVFDTGS
SNLWVPSIHC KLLDIACWIH HKYNSDKSST YVKNGTSFDI HYGSGSLSGY
LSQDTVSVPC QSASSASALG GVKVERQVFG EATKQPGITF IAAKFDGILG
MAYPRISVNN VLPVFDNLMQ QKLVDQNIFS FYLSRDPDAQ PGGELMLGGT
DSKYYKGSLS YLNVTRKAYW QVHLDQVEVA SGLTLCKEGC EAIVDTGTSL
MVGPVDEVRE LQKAIGAVPL IQGEYMIPCE KVSTLPAITL KLGGKGYKLS
PEDYTLKVSQ AGKTLCLSGF MGMDIPPPSG PLWILGDVFI GRYYTVFDRD
NNRVGFAEAA RL

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Human CTSD ELISA Kit