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Alternative name
Thymidine phosphorylase ELISA KIT; Gliostatin ELISA KIT; Platelet-derived endothelial cell growth factor ELISA KIT; PD-ECGF ELISA KIT; TdRPaseTYMP ELISA KIT; ECGF1 ELISA KIT; TP ELISA KIT; PD-ECGF ELISA KIT
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Catalog
E070089
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species
Mouse
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GeneTP
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Thymidine Phosphorylase (TP).
No significant cross-reactivity or interference between Thymidine Phosphorylase (TP) and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityTypically less than 0.115ng/mL.
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Intended UseMouse TP ELISA Kit allows for the in vitro quantitative determination of TP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Thymidine Phosphorylase (TP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Thymidine Phosphorylase (TP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Thymidine Phosphorylase (TP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Thymidine Phosphorylase (TP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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