Human CTSB ELISA Kit

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  • Alternative name

    Human APP secretase ELISA Kit;Human APPS ELISA Kit;Human cathepsin B1 ELISA Kit;Human CPSB ELISA Kit;Human cathepsin B ELISA Kit;Human amyloid precursor protein secretase ELISA Kit;Human cysteine protease ELISA Kit;

  • Catalog
    E007008
  • species
    Human
  • GeneCTSB
  • Standard CurveHuman CTSB ELISA Kit
  • Other Species Mouse Ctsb ELISA KitBovine CTSB ELISA KitChicken CTSB ELISA KitMonkey CTSB ELISA KitPorcine CTSB ELISA KitRat Ctsb ELISA KitSheep CTSB ELISA Kit
  • SpecificityNatural and recombinant human Cathepsin B
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity< 5pg/ml
  • Intended UseHuman CTSB ELISA Kit allows for the in vitro quantitative determination of CTSB , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: human Cathepsin B ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Cathepsin B has been precoated onto 96-well plates. Standards(NSO,R18-I339) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Cathepsin B is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Cathepsin B amount of sample captured in plate. Background: Cathepsin B is an enzymatic protein belonging to the peptidase or protease families. In humans, it is coded by the CTSB gene.1, 2 And this gene is mapped to chromosome 8p22.3 The protein encoded by this gene is a lysosomalcysteine proteinase composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. It is a member of the peptidase C1 family. Cathepsin B was once suspected as a candidate protease participating in the conversion of beta-amyloid precursor protein into the amyloid plaques found in Alzheimer's disease patients. However, this function is now known to be mediated by BACE1 protease. It is now thought that cathepsin B can degrade beta-amyloid precursor protein into harmless fragments. Thus, it is conceivable cathepsin B may play a pivotal role in the natural defense against Alzheimer's disease.4 Overexpression ofcathepsin B has been associated with esophageal adenocarcinoma and other tumors. At least five transcript variants encoding the same protein have been found for this gene. The standard product used in this kit is recombinant human Cathepsin B with the molecular mass of 37KDa.
  • Human Cathepsin B heavy chain Protein information
  • Uniprot ID CATB_HUMAN
  • Uniprot AC P07858; B3KQR5; B3KRR5; Q503A6; Q96D87;
  • UniGene Hs.520898;
  • GeneID 1508
  • KEGG hsa:1508;
  • Human Cathepsin B heavy chain Protein SEQUENCE
  • SEQUENCE 339 AA; 37822 MW; 0FC818EA4C1F6D90 CRC64;

    MWQLWASLCC LLVLANARSR PSFHPLSDEL VNYVNKRNTT WQAGHNFYNV

    DMSYLKRLCG TFLGGPKPPQ RVMFTEDLKL PASFDAREQW PQCPTIKEIR

    DQGSCGSCWA FGAVEAISDR ICIHTNAHVS VEVSAEDLLT CCGSMCGDGC

    NGGYPAEAWN FWTRKGLVSG GLYESHVGCR PYSIPPCEHH VNGSRPPCTG

    EGDTPKCSKI CEPGYSPTYK QDKHYGYNSY SVSNSEKDIM AEIYKNGPVE

    GAFSVYSDFL LYKSGVYQHV TGEMMGGHAI RILGWGVENG TPYWLVANSW

    NTDWGDNGFF KILRGQDHCG IESEVVAGIP RTDQYWEKI

  • UCSC uc003wun.4; human.;


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