Mouse Tslp ELISA Kit

Alternative name
Mouse thymic stroma-derived lymphopoietin ELISA Kit;Mouse thymic stromal lymphopoietin ELISA Kit;
Catalog
E070071
species
Mouse
GeneTslp
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 pg/mL.
Intended UseMouse Tslp ELISA Kit allows for the in vitro quantitative determination of Tslp , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyImmunology
Product Description
specificalPrinciple of the Assay: TSLP ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for TSLP. Standards or samples are then added to the microtiter plate wells and TSLP if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of TSLP present in the sample, a standardized preparation of horseradish peroxidase (HRP) -conjugated polyclonal antibody, specific for TSLP are added to each well to "sandwich" the TSLP immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain TSLP and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TSLP concentration in each sample is interpolated from this standard curve.
Mouse Thymic stromal lymphopoietin Protein information
Uniprot ID TSLP_MOUSE
Uniprot AC Q9JIE6;
UniGene Mm.143716;
GeneID 53603
KEGG mmu:53603;
Mouse Thymic stromal lymphopoietin Protein SEQUENCE
SEQUENCE 140 AA; 16152 MW; 5B6D3196E73BAA8B CRC64;
MVLLRSLFIL QVLVRMGLTY NFSNCNFTSI TKIYCNIIFH DLTGDLKGAK
FEQIEDCESK PACLLKIEYY TLNPIPGCPS LPDKTFARRT REALNDHCPG
YPETERNDGT QEMAQEVQNI CLNQTSQILR LWYSFMQSPE
UCSC uc008ejl.2; mouse.;

Mouse Tslp ELISA Kit

Mouse thymic stroma-derived lymphopoietin ELISA Kit;Mouse thymic stromal lymphopoietin ELISA Kit;

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