Mouse TSPAN30 ELISA Kit

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  • Catalog
    E069817
  • species
    Mouse
  • GeneTSPAN30
  • Standard CurveMouse TSPAN30 ELISA Kit
  • Other Species Human TSPAN30/CD63 ELISA KitHuman TSPAN30 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Tetraspanin 30 (TSPAN30). No significant cross-reactivity or interference between Tetraspanin 30 (TSPAN30) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.065ng/mL.
  • Intended UseMouse TSPAN30 ELISA Kit allows for the in vitro quantitative determination of TSPAN30 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Tetraspanin 30 (TSPAN30). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Tetraspanin 30 (TSPAN30). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Tetraspanin 30 (TSPAN30), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Tetraspanin 30 (TSPAN30) in the samples is then determined by comparing the O.D. of the samples to the standard curve.



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