Human COMT ELISA Kit

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  • Alternative name

    Human HEL-S-98n ELISA Kit;Human catechol-O-methyltransferase ELISA Kit;Human catechol O-methyltransferase ELISA Kit;Human catechol-O-methyltransferase isoform ELISA Kit;Human epididymis secretory sperm binding protein Li 98n ELISA Kit;Human testicular tissue protein Li 42 ELISA Kit;

  • Catalog
    E006959
  • species
    Human
  • GeneCOMT
  • Standard CurveHuman COMT ELISA Kit
  • Other Species Mouse Comt ELISA KitBovine COMT ELISA KitHorse COMT ELISA KitPorcine COMT ELISA KitRat Comt ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Catechol-O-Methyltransferase (COMT). No significant cross-reactivity or interference between Catechol-O-Methyltransferase (COMT) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.055ng/mL.
  • Intended UseHuman COMT ELISA Kit allows for the in vitro quantitative determination of COMT , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Catechol-O-Methyltransferase (COMT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Catechol-O-Methyltransferase (COMT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Catechol-O-Methyltransferase (COMT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Catechol-O-Methyltransferase (COMT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Catechol O-methyltransferase Protein information
  • Uniprot ID COMT_HUMAN
  • Uniprot AC P21964; A8MPV9; Q6IB07; Q6ICE6; Q9BWC7;
  • UniGene Hs.370408; Hs.693979; Hs.713616; Hs.738526;
  • GeneID 1312
  • KEGG hsa:1312;
  • Human Catechol O-methyltransferase Protein SEQUENCE
  • SEQUENCE 271 AA; 30037 MW; D2547A1C399AC758 CRC64;

    MPEAPPLLLA AVLLGLVLLV VLLLLLRHWG WGLCLIGWNE FILQPIHNLL

    MGDTKEQRIL NHVLQHAEPG NAQSVLEAID TYCEQKEWAM NVGDKKGKIV

    DAVIQEHQPS VLLELGAYCG YSAVRMARLL SPGARLITIE INPDCAAITQ

    RMVDFAGVKD KVTLVVGASQ DIIPQLKKKY DVDTLDMVFL DHWKDRYLPD

    TLLLEECGLL RKGTVLLADN VICPGAPDFL AHVRGSSCFE CTHYQSFLEY

    REVVDGLEKA IYKGPGSEAG P

  • UCSC uc002zqu.4; human. [P21964-1];


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