Human COMT ELISA Kit

Alternative name
Human HEL-S-98n ELISA Kit;Human catechol-O-methyltransferase ELISA Kit;Human catechol O-methyltransferase ELISA Kit;Human catechol-O-methyltransferase isoform ELISA Kit;Human epididymis secretory sperm binding protein Li 98n ELISA Kit;Human testicular tissue protein Li 42 ELISA Kit;
Catalog
E006959
species
Human
GeneCOMT
Standard Curve
Other Species Mouse Comt ELISA Kit Bovine COMT ELISA Kit Horse COMT ELISA Kit Porcine COMT ELISA Kit Rat Comt ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of Catechol-O-Methyltransferase (COMT). No significant cross-reactivity or interference between Catechol-O-Methyltransferase (COMT) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.055ng/mL.
Intended UseHuman COMT ELISA Kit allows for the in vitro quantitative determination of COMT , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Catechol-O-Methyltransferase (COMT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Catechol-O-Methyltransferase (COMT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Catechol-O-Methyltransferase (COMT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Catechol-O-Methyltransferase (COMT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Catechol O-methyltransferase Protein information
Uniprot ID COMT_HUMAN
Uniprot AC P21964; A8MPV9; Q6IB07; Q6ICE6; Q9BWC7;
UniGene Hs.370408; Hs.693979; Hs.713616; Hs.738526;
GeneID 1312
KEGG hsa:1312;
Human Catechol O-methyltransferase Protein SEQUENCE
SEQUENCE 271 AA; 30037 MW; D2547A1C399AC758 CRC64;
MPEAPPLLLA AVLLGLVLLV VLLLLLRHWG WGLCLIGWNE FILQPIHNLL
MGDTKEQRIL NHVLQHAEPG NAQSVLEAID TYCEQKEWAM NVGDKKGKIV
DAVIQEHQPS VLLELGAYCG YSAVRMARLL SPGARLITIE INPDCAAITQ
RMVDFAGVKD KVTLVVGASQ DIIPQLKKKY DVDTLDMVFL DHWKDRYLPD
TLLLEECGLL RKGTVLLADN VICPGAPDFL AHVRGSSCFE CTHYQSFLEY
REVVDGLEKA IYKGPGSEAG P
UCSC uc002zqu.4; human. [P21964-1];

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anti-COMT antibody

Category

Human COMT ELISA Kit

Human HEL-S-98n ELISA Kit;Human catechol-O-methyltransferase ELISA Kit;Human catechol O-methyltransferase ELISA Kit;Human catechol-O-methyltransferase isoform ELISA Kit;Human epididymis secretory sperm binding protein Li 98n ELISA Kit;Human testicular tissue protein Li 42 ELISA Kit;