Mouse Tbx3 ELISA Kit

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  • Alternative name

    Mouse T-box protein 3 ELISA Kit;Mouse D5Ertd189e ELISA Kit;Mouse T-box 3 ELISA Kit;Mouse T-box transcription factor TBX3 ELISA Kit;

  • Catalog
    E069439
  • species
    Mouse
  • GeneTbx3
  • Standard CurveMouse Tbx3 ELISA Kit
  • Other Species Human TBX3 ELISA KitChicken TBX3 ELISA KitRat Tbx3 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of TBX3. No significant cross-reactivity or interference between TBX3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between TBX3 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseMouse Tbx3 ELISA Kit allows for the in vitro quantitative determination of Tbx3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical
    Principle of the assay: TBX3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TBX3 antibody and an TBX3-HRP conjugate. The assay sample and buffer are incubated together with TBX3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TBX3 concentration since TBX3 from samples and TBX3-HRP conjugate compete for the anti-TBX3 antibody binding site. Since the number of sites is limited, as more sites are occupied by TBX3 from the sample, fewer sites are left to bind TBX3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TBX3 concentration in each sample is interpolated from this standard curve.
  • Mouse T-box transcription factor TBX3 Protein information
  • Uniprot ID TBX3_MOUSE
  • Uniprot AC P70324; E9QM97; Q60708; Q8BWH7;
  • UniGene Mm.219139;
  • GeneID 21386
  • KEGG mmu:21386;
  • Mouse T-box transcription factor TBX3 Protein SEQUENCE
  • SEQUENCE 741 AA; 79160 MW; B502FE817EFF124D CRC64;

    MSLSMRDPVI PGTSMAYHPF LPHRAPDFAM SAVLGHQPPF FPALTLPPNG

    AAALSLPGAL AKPIMDQLVG AAETGIPFSS LGPQAHLRPL KTMEPEEDVE

    DDPKVHLEAK ELWDQFHKRG TEMVITKSGR RMFPPFKVRC SGLDKKAKYI

    LLMDIIAADD CRYKFHNSRW MVAGKADPEM PKRMYIHPDS PATGEQWMSK

    VVTFHKLKLT NNISDKHGFT LAFPSDHATW QGNYSFGTQT ILNSMHKYQP

    RFHIVRANDI LKLPYSTFRT YLFPETEFIA VTAYQNDKIT QLKIDNNPFA

    KGFRDTGNGR REKRKQLTLQ SMRVFEERHK KETSDESSSE QAAFNCFAQA

    SSPAVSIVGT SNLKDLCPSE AESDAEAESK EEHGPEACDA AKISTTTAEE

    PGRDKGSPAT RAQLFPAEPS RARDTARLDK ASPDSRHSPA TISSSTRVPG

    ADERRSPGRE GPVATKVDEA RAIPAKDAFA PLSVQTDATA HLAQGPLPGL

    GFAPGLAGQQ FFNGHPLFLH PGQFAMGGAF SSMAAGMGPL LATVSGASTG

    VSGLESTAMA SAAAAQGLSG ASAATLPFHL QQHVLASQGL AMSPFGSLFP

    YPYTYMAAAA AASTAAASSS VHRHPFLNLN SMRPRLRYSP YSIPVPVPDS

    SSLLATALPS MASAAGPLDG KAAALAASPA SVAVDSGSEL NSRSSTLSSG

    SVSLSPKLCS EKEAATSELQ SIQRLVSGLE AKPDRSCSGS P

  • UCSC uc008zgu.2; mouse. [P70324-1];


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