Mouse Tbx3 ELISA Kit

Alternative name
Mouse T-box protein 3 ELISA Kit;Mouse D5Ertd189e ELISA Kit;Mouse T-box 3 ELISA Kit;Mouse T-box transcription factor TBX3 ELISA Kit;
Catalog
E069439
species
Mouse
GeneTbx3
SpecificityThis assay has high sensitivity and excellent specificity for detection of TBX3. No significant cross-reactivity or interference between TBX3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between TBX3 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseMouse Tbx3 ELISA Kit allows for the in vitro quantitative determination of Tbx3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description
specificalPrinciple of the assay: TBX3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-TBX3 antibody and an TBX3-HRP conjugate. The assay sample and buffer are incubated together with TBX3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the TBX3 concentration since TBX3 from samples and TBX3-HRP conjugate compete for the anti-TBX3 antibody binding site. Since the number of sites is limited, as more sites are occupied by TBX3 from the sample, fewer sites are left to bind TBX3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The TBX3 concentration in each sample is interpolated from this standard curve.
Mouse T-box transcription factor TBX3 Protein information
Uniprot ID TBX3_MOUSE
Uniprot AC P70324; E9QM97; Q60708; Q8BWH7;
UniGene Mm.219139;
GeneID 21386
KEGG mmu:21386;
Mouse T-box transcription factor TBX3 Protein SEQUENCE
SEQUENCE 741 AA; 79160 MW; B502FE817EFF124D CRC64;
MSLSMRDPVI PGTSMAYHPF LPHRAPDFAM SAVLGHQPPF FPALTLPPNG
AAALSLPGAL AKPIMDQLVG AAETGIPFSS LGPQAHLRPL KTMEPEEDVE
DDPKVHLEAK ELWDQFHKRG TEMVITKSGR RMFPPFKVRC SGLDKKAKYI
LLMDIIAADD CRYKFHNSRW MVAGKADPEM PKRMYIHPDS PATGEQWMSK
VVTFHKLKLT NNISDKHGFT LAFPSDHATW QGNYSFGTQT ILNSMHKYQP
RFHIVRANDI LKLPYSTFRT YLFPETEFIA VTAYQNDKIT QLKIDNNPFA
KGFRDTGNGR REKRKQLTLQ SMRVFEERHK KETSDESSSE QAAFNCFAQA
SSPAVSIVGT SNLKDLCPSE AESDAEAESK EEHGPEACDA AKISTTTAEE
PGRDKGSPAT RAQLFPAEPS RARDTARLDK ASPDSRHSPA TISSSTRVPG
ADERRSPGRE GPVATKVDEA RAIPAKDAFA PLSVQTDATA HLAQGPLPGL
GFAPGLAGQQ FFNGHPLFLH PGQFAMGGAF SSMAAGMGPL LATVSGASTG
VSGLESTAMA SAAAAQGLSG ASAATLPFHL QQHVLASQGL AMSPFGSLFP
YPYTYMAAAA AASTAAASSS VHRHPFLNLN SMRPRLRYSP YSIPVPVPDS
SSLLATALPS MASAAGPLDG KAAALAASPA SVAVDSGSEL NSRSSTLSSG
SVSLSPKLCS EKEAATSELQ SIQRLVSGLE AKPDRSCSGS P
UCSC uc008zgu.2; mouse. [P70324-1];

Category

Mouse Tbx3 ELISA Kit

Mouse T-box protein 3 ELISA Kit;Mouse D5Ertd189e ELISA Kit;Mouse T-box 3 ELISA Kit;Mouse T-box transcription factor TBX3 ELISA Kit;