Mouse SDF-1alpha/SDF1A ELISA Kit

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  • Alternative name

    Stromal cell-derived factor 1 ELISA KIT; C-X-C motif chemokine 12 ELISA KIT; Intercrine reduced in hepatomas ELISA KIT; IRH ELISA KIT; hIRH ELISA KIT; Pre-B cell growth-stimulating factor ELISA KIT; PBSFCXCL12 ELISA KIT; SDF1 ELISA KIT; SDF1A ELISA KIT; SDF1B ELISA KIT; SDF-1 ELISA KIT; hSDF-1 ELISA KIT; IRH ELISA KIT; hIRH ELISA KIT; PBSF ELISA KIT

  • Catalog
    E069043
  • species
    Mouse
  • GeneSDF-1alpha/SDF1A
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of mouse SDF-1alpha. No significant cross-reactivity or interference between mouse SDF-1alpha and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of mouse SDF-1alpha is typically less than 0.039 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined
  • Intended UseMouse SDF-1alpha/SDF1A ELISA Kit allows for the in vitro quantitative determination of SDF-1alpha/SDF1A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SDF-1alpha has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SDF-1alpha present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SDF-1alpha is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SDF-1alpha bound in the initial step. The color development is stopped and the intensity of the color is measured.




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