Human CARD10 ELISA Kit

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  • Alternative name

    Human CARD-containing MAGUK protein 3 ELISA Kit;Human carma 3 ELISA Kit;Human CARMA3 ELISA Kit;Human BIMP1 ELISA Kit;Human caspase recruitment domain family member 10 ELISA Kit;Human caspase recruitment domain-containing protein 10 ELISA Kit;Human Bcl10 binding protein and activator of NFKB ELISA Kit;Human CARD-containing MAGUK 3 protein ELISA Kit;

  • Catalog
    E006902
  • species
    Human
  • GeneCARD10
  • Standard CurveHuman CARD10 ELISA Kit
  • Other Species Mouse Card10 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of CARD10. No significant cross-reactivity or interference between CARD10 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CARD10 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL
  • Intended UseHuman CARD10 ELISA Kit allows for the in vitro quantitative determination of CARD10 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyCell Biology
  • Product Description
    specifical
    Intended Uses: This CARD10 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CARD10. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: CARD10 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CARD10 antibody and an CARD10-HRP conjugate. The assay sample and buffer are incubated together with CARD10-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CARD10 concentration since CARD10 from samples and CARD10-HRP conjugate compete for the anti-CARD10 antibody binding site. Since the number of sites is limited, as more sites are occupied by CARD10 from the sample, fewer sites are left to bind CARD10-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CARD10 concentration in each sample is interpolated from this standard curve.
  • Human Caspase recruitment domain-containing protein 10 Protein information
  • Uniprot ID CAR10_HUMAN
  • Uniprot AC Q9BWT7; Q14CQ8; Q5TFG6; Q8NC81; Q9UGR5; Q9UGR6; Q9Y3H0;
  • UniGene Hs.57973;
  • GeneID 29775
  • KEGG hsa:29775;
  • Human Caspase recruitment domain-containing protein 10 Protein SEQUENCE
  • SEQUENCE 1032 AA; 115931 MW; 298AAC132778376A CRC64;

    MPGRAEAGEA EEEAGAGSGS EAEEDALWER IEGVRHRLAR ALNPAKLTPY

    LRQCRVIDEQ DEEEVLSTYR FPCRVNRTGR LMDILRCRGK RGYEAFLEAL

    EFYYPEHFTL LTGQEPAQRC SMILDEEGPE GLTQFLMTEV RRLREARKSQ

    LQREQQLQAR GRVLEEERAG LEQRLRDQQQ AQERCQRLRE DWEAGSLELL

    RLKDENYMIA MRLAQLSEEK NSAVLRSRDL QLAVDQLKLK VSRLEEECAL

    LRRARGPPPG AEEKEKEKEK EKEPDNVDLV SELRAENQRL TASLRELQEG

    LQQEASRPGA PGSERILLDI LEHDWREAQD SRQELCQKLH AVQGELQWAE

    ELRDQYLQEM EDLRLKHRTL QKDCDLYKHR MATVLAQLEE IEKERDQAIQ

    SRDRIQLQYS QSLIEKDQYR KQVRGLEAER DELLTTLTSL EGTKALLEVQ

    LQRAQGGTCL KACASSHSLC SNLSSTWSLS EFPSPLGGPE ATGEAAVMGG

    PEPHNSEEAT DSEKEINRLS ILPFPPSAGS ILRRQREEDP APPKRSFSSM

    SDITGSVTLK PWSPGLSSSS SSDSVWPLGK PEGLLARGCG LDFLNRSLAI

    RVSGRSPPGG PEPQDKGPDG LSFYGDRWSG AVVRRVLSGP GSARMEPREQ

    RVEAAGLEGA CLEAEAQQRT LLWNQGSTLP SLMDSKACQS FHEALEAWAK

    GPGAEPFYIR ANLTLPERAD PHALCVKAQE ILRLVDSAYK RRQEWFCTRV

    DPLTLRDLDR GTVPNYQRAQ QLLEVQEKCL PSSRHRGPRS NLKKRALDQL

    RLVRPKPVGA PAGDSPDQLL LEPCAEPERS LRPYSLVRPL LVSALRPVVL

    LPECLAPRLI RNLLDLPSSR LDFQVCPAES LSGEELCPSS APGAPKAQPA

    TPGLGSRIRA IQESVGKKHC LLELGARGVR ELVQNEIYPI VIHVEVTEKN

    VREVRGLLGR PGWRDSELLR QCRGSEQVLW GLPCSWVQVP AHEWGHAEEL

    AKVVRGRILQ EQARLVWVEC GSSRGCPSSS EA

  • UCSC uc003asw.2; human. [Q9BWT7-1];



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