Mouse Srd5a2 ELISA Kit

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  • Alternative name

    Mouse 5 alpha-SR2 ELISA Kit;Mouse SR type 2 ELISA Kit;Mouse Steroid 5-alpha-reductase 2 ELISA Kit;Mouse S5AR 2 ELISA Kit;Mouse 5ART2 ELISA Kit;Mouse steroid 5 alpha-reductase 2 ELISA Kit;Mouse 3-oxo-5-alpha-steroid 4-dehydrogenase 2 ELISA Kit;Mouse steroid 5 alpha reductase type2 ELISA Kit;

  • Catalog
    E068975
  • species
    Mouse
  • GeneSrd5a2
  • Standard CurveMouse Srd5a2 ELISA Kit
  • Other Species Human SRD5A2 ELISA KitMonkey SRD5A2 ELISA KitPorcine SRD5A2 ELISA KitRat Srd5a2 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of SRD5a2. No significant cross-reactivity or interference between SRD5a2 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of SRD5a2 is typically less than 0.062ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding t
  • Intended UseMouse Srd5a2 ELISA Kit allows for the in vitro quantitative determination of Srd5a2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to SRD5a2. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to SRD5a2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain SRD5a2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of SRD5a2 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Mouse 3-oxo-5-alpha-steroid 4-dehydrogenase 2 Protein information
  • Uniprot ID S5A2_MOUSE
  • Uniprot AC Q99N99; Q3UTZ9;
  • UniGene Mm.38933;
  • GeneID 94224
  • KEGG mmu:94224;
  • Mouse 3-oxo-5-alpha-steroid 4-dehydrogenase 2 Protein SEQUENCE
  • SEQUENCE 254 AA; 28619 MW; 8D25019E8DC4DF47 CRC64;

    MPIVCHQVPV LAGSATLATM GTLILCFGKP ASYGKHSESV SSGVPLLPAR

    IAWFLQELPS FVVSVGMLAW QPRSLFGPPG NVLLGLFSAH YFHRTFIYSL

    LTRGRPLSAV IFLKATAFCI GNGLLQAYYL VYCAEYPEEW YTDMRFSVGV

    FFFILGMGIN IHSDCMLRQL RKPGEVIYRI PQGGLFTYVS GANFLGEIIE

    WMGYALATWS VPAFAFAFFT LCFLGMQAFY HHRFYLKMFK DYPKSRKALI

    PFIF

  • UCSC uc008dnt.1; mouse.;



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