Human CASP8 ELISA Kit

Alternative name
Human Casp-8 ELISA Kit;Human apoptotic cysteine protease ELISA Kit;Human apoptotic protease Mch-5 ELISA Kit;Human CAP4 ELISA Kit;Human FADD-homologous ICE/CED-3-like protease ELISA Kit;Human FADD-like ICE ELISA Kit;Human FLICE ELISA Kit;Human ICE-like apoptotic protease 5 ELISA Kit;Human MORT1-associated ced-3 homolog ELISA Kit;Human MACH ELISA Kit;Human MCH5 ELISA Kit;Human ALPS2B ELISA Kit;Human caspase 8 ELISA Kit;Human caspase-8 ELISA Kit;Human MACH-alpha-1/2/3 protein ELISA Kit;Human MACH-beta-1/2/3/4 protein ELISA Kit;Human caspase 8, apoptosis-related cysteine peptidase ELISA Kit;Human caspase 8, apoptosis-related cysteine protease ELISA Kit;
Catalog
E006888
species
Human
GeneCASP8
Standard Curve
Other Species Mouse Casp8 ELISA Kit Rat Casp8 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of Caspase 8 (CASP8). No significant cross-reactivity or interference between Caspase 8 (CASP8) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.057ng/mL.
Intended UseHuman CASP8 ELISA Kit allows for the in vitro quantitative determination of CASP8 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Caspase 8 (CASP8). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Caspase 8 (CASP8). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Caspase 8 (CASP8), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Caspase 8 (CASP8) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Caspase-8 subunit p10 Protein information
Uniprot ID CASP8_HUMAN
Uniprot AC Q96T22; Q9C0K4; Q9UQ81;
UniGene Hs.599762;
GeneID 841
KEGG hsa:841;
Human Caspase-8 subunit p10 Protein SEQUENCE
SEQUENCE 479 AA; 55391 MW; 7A5FEAA6B39B582F CRC64;
MDFSRNLYDI GEQLDSEDLA SLKFLSLDYI PQRKQEPIKD ALMLFQRLQE
KRMLEESNLS FLKELLFRIN RLDLLITYLN TRKEEMEREL QTPGRAQISA
YRVMLYQISE EVSRSELRSF KFLLQEEISK CKLDDDMNLL DIFIEMEKRV
ILGEGKLDIL KRVCAQINKS LLKIINDYEE FSKERSSSLE GSPDEFSNGE
ELCGVMTISD SPREQDSESQ TLDKVYQMKS KPRGYCLIIN NHNFAKAREK
VPKLHSIRDR NGTHLDAGAL TTTFEELHFE IKPHDDCTVE QIYEILKIYQ
LMDHSNMDCF ICCILSHGDK GIIYGTDGQE APIYELTSQF TGLKCPSLAG
KPKVFFIQAC QGDNYQKGIP VETDSEEQPY LEMDLSSPQT RYIPDEADFL
LGMATVNNCV SYRNPAEGTW YIQSLCQSLR ERCPRGDDIL TILTEVNYEV
SNKDDKKNMG KQMPQPTFTL RKKLVFPSD
UCSC uc002uxo.2; human. [Q14790-1];

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Human CASP8 ELISA Kit