Mouse Spon1 ELISA Kit

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  • Alternative name

    Mouse F-spondin ELISA Kit;Mouse AI666765 ELISA Kit;Mouse AW455831 ELISA Kit;Mouse BC020531 ELISA Kit;Mouse D330035F22Rik ELISA Kit;Mouse FSP ELISA Kit;Mouse spondin 1, (f-spondin) extracellular matrix protein ELISA Kit;Mouse spondin-1 ELISA Kit;

  • Catalog
    E068813
  • species
    Mouse
  • GeneSpon1
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of mouse SPON1. No significant cross-reactivity or interference between mouse SPON1 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of mouse SPON1 is typically less than 0.039 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the m
  • Intended UseMouse Spon1 ELISA Kit allows for the in vitro quantitative determination of Spon1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SPON1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SPON1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SPON1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SPON1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Mouse Spondin-1 Protein information
  • Uniprot ID SPON1_MOUSE
  • Uniprot AC Q8VCC9; Q8K2Q8;
  • UniGene Mm.334160;
  • GeneID 233744
  • KEGG mmu:233744;
  • Mouse Spondin-1 Protein SEQUENCE
  • SEQUENCE 807 AA; 90821 MW; 3116DF867F4AE563 CRC64;

    MRLSPVSLRL SRGPALLALA LPLAAALAFS DETLDKVTKS EGYCSRILRA

    QGTRREGYTE FSLRVEGDPD FYKPGSSYRV TLSAAPPSYF RGFTLIALKE

    NQEGDKEEDH AGTFQIIDEE ETQFMSNCPV AVTESTPRRR TRIQVFWIAP

    PTGTGCVILK ASIVQKRIIY FQDEGSLTKK LCEQDPTLDG VTDRPILDCC

    ACGTAKYRLT FYGNWSEKTH PKDYPRRANH WSAIIGGSHS KNYVLWEYGG

    YASEGVKQVA ELGSPVKMEE EIRQQSDEVL TVIKAKAQWP AWQPVNVRAA

    PSAEFSVDRT RHLMSFLTMM GPSPDWNVGL SAEDLCTKEC GWVQKVVQDL

    IPWDAGTDSG VTYESPNKPT IPQEKIRPLT SLDHPQSPFY DPEGGSITQV

    ARVVIERIAR KGEQCNIVPD NVDDIVADLA PEEKDEDDTP ETCIYSNWSP

    WSACSSSTCE KGKRMRQRML KAQLDLSVPC PDTQDFQPCM GPGCSDEDGS

    TCTMSEWITW SPCSVSCGMG MRSRERYVKQ FPEDGSVCML PTEETEKCTV

    NEECSPSSCL VTEWGEWDDC SATCGMGMKK RHRMVKMSPA DGSMCKAETS

    QAEKCMMPEC HTIPCLLSPW SEWSDCSVTC GKGMRTRQRM LKSLAELGDC

    NEDLEQAEKC MLPECPIDCE LSEWSQWSEC NKSCGKGHMI RTRTIQMEPQ

    FGGVPCPETV QRKKCRTRKC LRSPSVQKLR WREARESRRS EQLREESDGE

    QFPGCRMRPW TAWSECTKLC GGGIQERYMT VKKRFKSSQF TSCKDKKEIR

    ACNVHPC

  • UCSC uc009jhv.2; mouse.;



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