Mouse Spa17 ELISA Kit

Alternative name
Mouse sperm autoantigenic protein 17 ELISA Kit;Mouse Sp17 ELISA Kit;Mouse sperm surface protein Sp17 ELISA Kit;Mouse band 34 ELISA Kit;Mouse sperm protein 17 ELISA Kit;
Catalog
E068692
species
Mouse
GeneSpa17
SpecificityThis assay recognizes recombinant and natural mouse Sperm surface protein Sp17. No significant cross-reactivity or interference was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Intended UseMouse Spa17 ELISA Kit allows for the in vitro quantitative determination of Spa17 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Sperm surface protein Sp17. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Sperm surface protein Sp17 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Sperm surface protein Sp17, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Sperm surface protein Sp17 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Mouse Sperm surface protein Sp17 Protein information
Uniprot ID SP17_MOUSE
Uniprot AC Q62252;
UniGene Mm.196345; Mm.8637;
GeneID 20686
KEGG mmu:20686;
Mouse Sperm surface protein Sp17 Protein SEQUENCE
SEQUENCE 149 AA; 17296 MW; C7E05D111D6AF0DC CRC64;
MSIPFSNTHY RIPQGFGNLL EGLTREILRE QPDNIPAFAA AYFENLLEKR
EKTSFDPAEW GAKVEDRFYN NHAFKEQEQV EKCEQELAKS SGREETPVTP
FEESTEEERE QEEAAALKIQ SLFRGHVARE EVKKMKSDKN ENLKEEADN
UCSC uc009ovd.2; mouse.;

Mouse Spa17 ELISA Kit

Mouse sperm autoantigenic protein 17 ELISA Kit;Mouse Sp17 ELISA Kit;Mouse sperm surface protein Sp17 ELISA Kit;Mouse band 34 ELISA Kit;Mouse sperm protein 17 ELISA Kit;

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