Human CASP14 ELISA Kit

Alternative name
Human CASP-14 ELISA Kit;Human ARCI12 ELISA Kit;Human caspase 14 ELISA Kit;Human caspase-14 ELISA Kit;Human apoptosis-related cysteine protease ELISA Kit;Human caspase 14, apoptosis-related cysteine peptidase ELISA Kit;Human caspase 14, apoptosis-related cysteine protease ELISA Kit;
Catalog
E006857
species
Human
GeneCASP14
Standard Curve
Other Species Mouse Casp14 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of CASP14. No significant cross-reactivity or interference between CASP14 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CASP14 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman CASP14 ELISA Kit allows for the in vitro quantitative determination of CASP14 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyCell Biology
Product Description
specificalIntended Uses: This CASP14 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CASP14. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||CASP14 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CASP14 antibody and an CASP14-HRP conjugate. The assay sample and buffer are incubated together with CASP14-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CASP14 concentration since CASP14 from samples and CASP14-HRP conjugate compete for the anti-CASP14 antibody binding site. Since the number of sites is limited, as more sites are occupied by CASP14 from the sample, fewer sites are left to bind CASP14-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CASP14 concentration in each sample is interpolated from this standard curve.
Human Caspase-14 subunit p8, intermediate form Protein information
Uniprot ID CASPE_HUMAN
Uniprot AC P31944; O95823; Q3SYC9;
UniGene Hs.466057;
GeneID 23581
KEGG hsa:23581;
Human Caspase-14 subunit p8, intermediate form Protein SEQUENCE
SEQUENCE 242 AA; 27680 MW; E539FB7E8DD808A2 CRC64;
MSNPRSLEEE KYDMSGARLA LILCVTKARE GSEEDLDALE HMFRQLRFES
TMKRDPTAEQ FQEELEKFQQ AIDSREDPVS CAFVVLMAHG REGFLKGEDG
EMVKLENLFE ALNNKNCQAL RAKPKVYIIQ ACRGEQRDPG ETVGGDEIVM
VIKDSPQTIP TYTDALHVYS TVEGYIAYRH DQKGSCFIQT LVDVFTKRKG
HILELLTEVT RRMAEAELVQ EGKARKTNPE IQSTLRKRLY LQ
UCSC uc010dzv.3; human.;

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Human CASP14 ELISA Kit

Human CASP-14 ELISA Kit;Human ARCI12 ELISA Kit;Human caspase 14 ELISA Kit;Human caspase-14 ELISA Kit;Human apoptosis-related cysteine protease ELISA Kit;Human caspase 14, apoptosis-related cysteine peptidase ELISA Kit;Human caspase 14, apoptosis-related cysteine protease ELISA Kit;