Human CASP1 ELISA Kit

Alternative name
Human CASP-1 ELISA Kit;Human Interleukin-1 beta convertase ELISA Kit;Human IL-1BC ELISA Kit;Human Interleukin-1 beta-converting enzyme ELISA Kit;Human ICE ELISA Kit;Human IL-1 beta-converting enzyme ELISA Kit;Human P45 ELISA Kit;Human IL1BC ELISA Kit;Human IL1BCE ELISA Kit;Human caspase 1 ELISA Kit;Human caspase-1 ELISA Kit;Human CASP1 nirs variant 1 ELISA Kit;Human IL1B-convertase ELISA Kit;Human caspase 1, apoptosis-related cysteine peptidase ELISA Kit;Human interleukin 1, beta, convertase ELISA Kit;Human interleukin 1-B converting enzyme ELISA Kit;
Catalog
E006844
species
Human
GeneCASP1
SpecificityThis assay has high sensitivity and excellent specificity for detection of Caspase 1 (CASP1). No significant cross-reactivity or interference between Caspase 1 (CASP1) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.113ng/mL.
Intended UseHuman CASP1 ELISA Kit allows for the in vitro quantitative determination of CASP1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Caspase 1 (CASP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Caspase 1 (CASP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Caspase 1 (CASP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Caspase 1 (CASP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Caspase-1 subunit p10 Protein information
Uniprot ID CASP1_HUMAN
Uniprot AC SCDVEEIF RKVRFSFEQP DGRAQMPTTE RVTLTRCFYL FPGH
UniGene Hs.2490;
GeneID 834
KEGG hsa:834;
Human Caspase-1 subunit p10 Protein SEQUENCE
SEQUENCE 404 AA; 45159 MW; ABF33CF33CC71584 CRC64;
MADKVLKEKR KLFIRSMGEG TINGLLDELL QTRVLNKEEM EKVKRENATV
MDKTRALIDS VIPKGAQACQ ICITYICEED SYLAGTLGLS ADQTSGNYLN
MQDSQGVLSS FPAPQAVQDN PAMPTSSGSE GNVKLCSLEE AQRIWKQKSA
EIYPIMDKSS RTRLALIICN EEFDSIPRRT GAEVDITGMT MLLQNLGYSV
DVKKNLTASD MTTELEAFAH RPEHKTSDST FLVFMSHGIR EGICGKKHSE
QVPDILQLNA IFNMLNTKNC PSLKDKPKVI IIQACRGDSP GVVWFKDSVG
VSGNLSLPTT EEFEDDAIKK AHIEKDFIAF CSSTPDNVSW RHPTMGSVFI
GRLIEHMQEY ACSCDVEEIF RKVRFSFEQP DGRAQMPTTE RVTLTRCFYL
FPGH
UCSC uc001pig.5; human. [P29466-1];

Human CASP1 ELISA Kit

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