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Mouse Smurf1 ELISA Kit

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  • Alternative name

    Mouse HECT-type E3 ubiquitin transferase SMURF1 ELISA Kit;Mouse Smad ubiquitination regulatory factor 1 ELISA Kit;Mouse SMAD-specific E3 ubiquitin-protein ligase 1 ELISA Kit;Mouse 4930431E10Rik ELISA Kit;Mouse mKIAA1625 ELISA Kit;Mouse SMAD specific E3 ubiquitin protein ligase 1 ELISA Kit;Mouse E3 ubiquitin-protein ligase SMURF1 ELISA Kit;
  • Catalog
    E067920
  • species
    Mouse
  • GeneSmurf1
  • Other Species Human SMURF1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of mouse SMURF1. No significant cross-reactivity or interference between mouse SMURF1 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of mouse SMURF1 is typically less than 7.8 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the me
  • Intended UseMouse Smurf1 ELISA Kit allows for the in vitro quantitative determination of Smurf1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for SMURF1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any SMURF1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for SMURF1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of SMURF1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Mouse E3 ubiquitin-protein ligase SMURF1 Protein information
  • Uniprot ID SMUF1_MOUSE
  • Uniprot AC Q9CUN6; Q3U412; Q8K300;
  • UniGene Mm.27735;
  • GeneID 75788
  • KEGG mmu:75788;
  • Mouse E3 ubiquitin-protein ligase SMURF1 Protein SEQUENCE

  • SEQUENCE 731 AA; 83356 MW; F0A7A1B019CC9D3E CRC64;

    MSNPGTRRNG SSIKIRLTVL CAKNLAKKDF FRLPDPFAKI VVDGSGQCHS

    TDTVKNTLDP KWNQHYDLYV GKTDSITISV WNHKKIHKKQ GAGFLGCVRL

    LSNAISRLKD TGYQRLDLCK LNPSDTDAVR GQIVVSLQTR DRIGGGGSVV

    DCRGLLENEG TVYEDSGPGR PLSCLMEEPA PYTDGTGAAA GGGNCRFVES

    PSQDQRLLVQ RLRNPEVRGP LQTPQNRPHG HQSPELPEGY EQRTTVQGQV

    YFLHTQTGVS TWHDPRIPRD LNSVNCDELG PLPPGWEVRS TVSGRIYFVD

    HNNRTTQFTD PRLHHIMNHQ CQLKEPSQPL QLPSEGSVED EELPAQRYER

    DLVQKLKVLR HELSLQQPQA GHCRIEVSRE EIFEESYRQI MKMRPKDLKK

    RLMVKFRGEE GLDYGGVARE WLYLLCHEML NPYYGLFQYS TDNIYTLQIN

    PDSSINPDHL SYFHFVGRIM GLAVFHGHYI NGGFTVPFYK QLLGKPIQLS

    DLESVDPELH KSLVWILEND ITPVLDHTFC VEHNAFGRIL QHELKPNGRN

    VPVTEENKKE YVRLYVNWRF MRGIEAQFLA LQKGFNELIP QHLLKPFDQK

    ELELIIGGLD KIDLNDWKSN TRLKHCVADS NIVRWFWQAV ETFDEERRAR

    LLQFVTGSTR VPLQGFKALQ GSTGAAGPRL FTIHLIDANT DNLPKAHTCF

    NRIDIPPYES YEKLYEKLLT AVEETCGFAV E

  • UCSC uc009alv.1; mouse.;


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