Human CILP ELISA Kit

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  • Alternative name

    Human CILP-1 ELISA Kit;Human Cartilage intermediate-layer protein ELISA Kit;Human HsT18872 ELISA Kit;Human cartilage intermediate layer protein ELISA Kit;Human cartilage intermediate layer protein 1 ELISA Kit;Human cartilage intermediate layer protein 1 C1 ELISA Kit;Human cartilage intermediate layer protein 1 C2 ELISA Kit;Human cartilage intermediate layer protein, nucleotide pyrophosphohydrolase ELISA Kit;

  • Catalog
    E006772
  • species
    Human
  • GeneCILP
  • Other Species Mouse Cilp ELISA KitPorcine CILP ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of CILP. No significant cross-reactivity or interference between CILP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CILP and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman CILP ELISA Kit allows for the in vitro quantitative determination of CILP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical
    Principle of the Assay: CILP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CILP antibody and an CILP-HRP conjugate. The assay sample and buffer are incubated together with CILP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CILP concentration since CILP from samples and CILP-HRP conjugate compete for the anti-CILP antibody binding site. Since the number of sites is limited, as more sites are occupied by CILP from the sample, fewer sites are left to bind CILP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CILP concentration in each sample is interpolated from this standard curve.
  • Human Cartilage intermediate layer protein 1 C2 Protein information
  • Uniprot ID CILP1_HUMAN
  • Uniprot AC EEAPPSAA HFRFYQIEGD RYDYNTVPFN EDDPMSWTED YLAWWPKPME FRACYIKVKI
  • UniGene Hs.442180;
  • GeneID 8483
  • KEGG hsa:8483;
  • Human Cartilage intermediate layer protein 1 C2 Protein SEQUENCE
  • SEQUENCE 1184 AA; 132565 MW; 190B0316404D3B84 CRC64;

    MVGTKAWVFS FLVLEVTSVL GRQTMLTQSV RRVQPGKKNP SIFAKPADTL

    ESPGEWTTWF NIDYPGGKGD YERLDAIRFY YGDRVCARPL RLEARTTDWT

    PAGSTGQVVH GSPREGFWCL NREQRPGQNC SNYTVRFLCP PGSLRRDTER

    IWSPWSPWSK CSAACGQTGV QTRTRICLAE MVSLCSEASE EGQHCMGQDC

    TACDLTCPMG QVNADCDACM CQDFMLHGAV SLPGGAPASG AAIYLLTKTP

    KLLTQTDSDG RFRIPGLCPD GKSILKITKV KFAPIVLTMP KTSLKAATIK

    AEFVRAETPY MVMNPETKAR RAGQSVSLCC KATGKPRPDK YFWYHNDTLL

    DPSLYKHESK LVLRKLQQHQ AGEYFCKAQS DAGAVKSKVA QLIVIASDET

    PCNPVPESYL IRLPHDCFQN ATNSFYYDVG RCPVKTCAGQ QDNGIRCRDA

    VQNCCGISKT EEREIQCSGY TLPTKVAKEC SCQRCTETRS IVRGRVSAAD

    NGEPMRFGHV YMGNSRVSMT GYKGTFTLHV PQDTERLVLT FVDRLQKFVN

    TTKVLPFNKK GSAVFHEIKM LRRKKPITLE AMETNIIPLG EVVGEDPMAE

    LEIPSRSFYR QNGEPYIGKV KASVTFLDPR NISTATAAQT DLNFINDEGD

    TFPLRTYGMF SVDFRDEVTS EPLNAGKVKV HLDSTQVKMP EHISTVKLWS

    LNPDTGLWEE EGDFKFENQR RNKREDRTFL VGNLEIRERR LFNLDVPESR

    RCFVKVRAYR SERFLPSEQI QGVVISVINL EPRTGFLSNP RAWGRFDSVI

    TGPNGACVPA FCDDQSPDAY SAYVLASLAG EELQAVESSP KFNPNAIGVP

    QPYLNKLNYR RTDHEDPRVK KTAFQISMAK PRPNSAEESN GPIYAFENLR

    ACEEAPPSAA HFRFYQIEGD RYDYNTVPFN EDDPMSWTED YLAWWPKPME

    FRACYIKVKI VGPLEVNVRS RNMGGTHRQT VGKLYGIRDV RSTRDRDQPN

    VSAACLEFKC SGMLYDQDRV DRTLVKVIPQ GSCRRASVNP MLHEYLVNHL

    PLAVNNDTSE YTMLAPLDPL GHNYGIYTVT DQDPRTAKEI ALGRCFDGTS

    DGSSRIMKSN VGVALTFNCV ERQVGRQSAF QYLQSTPAQS PAAGTVQGRV

    PSRRQQRASR GGQRQGGVVA SLRFPRVAQQ PLIN

  • UCSC uc002aon.3; human.;




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