Human CRTAP ELISA Kit

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  • Alternative name

    Human CASP ELISA Kit;Human LEPREL3 ELISA Kit;Human OI7 ELISA Kit;Human P3H5 ELISA Kit;Human cartilage associated protein ELISA Kit;Human cartilage-associated protein ELISA Kit;Human leprecan-like 3 ELISA Kit;Human prolyl 3-hydroxylase family member 5 (non-enzymatic) ELISA Kit;

  • Catalog
    E006764
  • species
    Human
  • GeneCRTAP
  • Standard CurveHuman CRTAP ELISA Kit
  • Other Species Mouse Crtap ELISA KitChicken CRTAP ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Cartilage Associated Protein (CRTAP). No significant cross-reactivity or interference between Cartilage Associated Protein (CRTAP) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.061ng/mL.
  • Intended UseHuman CRTAP ELISA Kit allows for the in vitro quantitative determination of CRTAP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cartilage Associated Protein (CRTAP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cartilage Associated Protein (CRTAP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cartilage Associated Protein (CRTAP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Cartilage Associated Protein (CRTAP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Cartilage-associated protein Protein information
  • Uniprot ID CRTAP_HUMAN
  • Uniprot AC O75718; B2RBL6;
  • UniGene Hs.517888;
  • GeneID 10491
  • KEGG hsa:10491;
  • Human Cartilage-associated protein Protein SEQUENCE
  • SEQUENCE 401 AA; 46562 MW; 4BEED4089195456F CRC64;

    MEPGRRGAAA LLALLCVACA LRAGRAQYER YSFRSFPRDE LMPLESAYRH

    ALDKYSGEHW AESVGYLEIS LRLHRLLRDS EAFCHRNCSA APQPEPAAGL

    ASYPELRLFG GLLRRAHCLK RCKQGLPAFR QSQPSREVLA DFQRREPYKF

    LQFAYFKANN LPKAIAAAHT FLLKHPDDEM MKRNMAYYKS LPGAEDYIKD

    LETKSYESLF IRAVRAYNGE NWRTSITDME LALPDFFKAF YECLAACEGS

    REIKDFKDFY LSIADHYVEV LECKIQCEEN LTPVIGGYPV EKFVATMYHY

    LQFAYYKLND LKNAAPCAVS YLLFDQNDKV MQQNLVYYQY HRDTWGLSDE

    HFQPRPEAVQ FFNVTTLQKE LYDFAKENIM DDDEGEVVEY VDDLLELEET

    S

  • UCSC uc003cfl.5; human.;


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