Mouse Shbg ELISA Kit

Alternative name
Mouse SHBG ELISA Kit;Mouse sex steroid-binding protein ELISA Kit;Mouse SBP ELISA Kit;Mouse testis-specific androgen-binding protein ELISA Kit;Mouse ABP ELISA Kit;Mouse sex hormone binding globulin ELISA Kit;Mouse sex hormone-binding globulin ELISA Kit;
Catalog
E067605
species
Mouse
GeneShbg
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 pg/mL.
Intended UseMouse Shbg ELISA Kit allows for the in vitro quantitative determination of Shbg , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description
specificalPrinciple of the Assay: SHBG ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SHBG antibody and an SHBG-HRP conjugate. The assay sample and buffer are incubated together with SHBG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SHBG concentration since SHBG from samples and SHBG-HRP conjugate compete for the anti-SHBG antibody binding site. Since the number of sites is limited, as more sites are occupied by SHBG from the sample, fewer sites are left to bind SHBG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SHBG concentration in each sample is interpolated from this standard curve.
Mouse Sex hormone-binding globulin Protein information
Uniprot ID SHBG_MOUSE
Uniprot AC P97497; Q0VB52;
UniGene Mm.1431;
GeneID 20415
KEGG mmu:20415;
Mouse Sex hormone-binding globulin Protein SEQUENCE
SEQUENCE 403 AA; 44816 MW; 5198DDD1CDC23530 CRC64;
MEKRDSVALH WRLLLLLLLL MPPPTHQGRA LRHIDPIQSA QDPPAKYLSN
GPGQEPVMVM TIDLTKISKP HSSFEFRTWD PEGVIFYGDT NTEDDWFLLG
LRAGQLEIQL HNAWARLTVG FGPRLDDGRW HPVELKMNGD SLLLWVDGKE
MLCLRQISAS LADHSQRSMR IALGGLLLPT SKLRFPLVPA LDGCIRRDIW
LGHQAQLSAS PRTSLGNCDV DLQPGLFFPP GTHAEFSLQD IPQPHADPWT
FSLELGFKLV DGSGQLLALG TGTNSSWLNI HLQNQSVVLS SEAEPKVVLP
LDVGLPLQLT LDRVKVVLSQ GPKMEVLSMS LLRPASLWRL WSHPQGHLSL
GALPGESSSA SFCLSDFWVQ GQRLDIDQAL SRSQDIWTHS CPQRPSNDTR
TSH
UCSC uc007jqp.2; mouse.;

Category

Mouse Shbg ELISA Kit

Mouse SHBG ELISA Kit;Mouse sex steroid-binding protein ELISA Kit;Mouse SBP ELISA Kit;Mouse testis-specific androgen-binding protein ELISA Kit;Mouse ABP ELISA Kit;Mouse sex hormone binding globulin ELISA Kit;Mouse sex hormone-binding globulin ELISA Kit;