SpecificityThis assay recognizes recombinant and natural mouse Serine/arginine-rich splicing factor 3. No significant cross-reactivity or interference was observed.
Intended UseMouse Srsf3 ELISA Kit allows for the in vitro quantitative determination of Srsf3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description specificalPrinciple of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Serine/arginine-rich splicing factor 3. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Serine/arginine-rich splicing factor 3 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Serine/arginine-rich splicing factor 3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Serine/arginine-rich splicing factor 3 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Mouse Serine/arginine-rich splicing factor 3 Protein information
Uniprot ID
SRSF3_MOUSE
Uniprot AC
P84104; O08831; P23152; Q564E9;
UniGene
Mm.473125; Mm.486331; Mm.6787;
GeneID
20383
KEGG
mmu:20383;
Mouse Serine/arginine-rich splicing factor 3 Protein SEQUENCE