-
Alternative name
Serine protease inhibitor Kazal-type 5 ELISA KIT; Lympho-epithelial Kazal-type-related inhibitor ELISA KIT; LEKTISPINK5 ELISA KIT; LEKTI ELISA KIT
-
Catalog
E067345
-
species
Mouse
-
GeneSPINK5
-
SpecificityThis assay has high sensitivity and excellent specificity for detection of Serine Peptidase Inhibitor Kazal Type 5 (SPINK5).
No significant cross-reactivity or interference between Serine Peptidase Inhibitor Kazal Type 5 (SPINK5) and analogues was observed.
-
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
-
SensitivityTypically less than 29pg/mL.
-
Intended UseMouse SPINK5 ELISA Kit allows for the in vitro quantitative determination of SPINK5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
-
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
-
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Serine Peptidase Inhibitor Kazal Type 5 (SPINK5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Serine Peptidase Inhibitor Kazal Type 5 (SPINK5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Serine Peptidase Inhibitor Kazal Type 5 (SPINK5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Serine Peptidase Inhibitor Kazal Type 5 (SPINK5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
-