Human CPT1A ELISA Kit

Alternative name
Human CPT1-L ELISA Kit;Human carnitine O-palmitoyltransferase I, liver isoform ELISA Kit;Human CPT I ELISA Kit;Human CPTI-L ELISA Kit;Human carnitine palmitoyltransferase 1A ELISA Kit;Human CPT1 ELISA Kit;Human L-CPT1 ELISA Kit;Human carnitine O-palmitoyltransferase 1, liver isoform ELISA Kit;Human carnitine palmitoyltransferase 1A (liver) ELISA Kit;Human carnitine palmitoyltransferase I, liver ELISA Kit;
Catalog
E006734
species
Human
GeneCPT1A
Other Species Mouse Cpt1a ELISA Kit Horse CPT1A ELISA Kit Rat Cpt1a ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of CPT1A. No significant cross-reactivity or interference between CPT1A and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CPT1A and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman CPT1A ELISA Kit allows for the in vitro quantitative determination of CPT1A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalIntended Uses||This CPT1A ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CPT1A. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: CPT1A ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-CPT1A antibody and an CPT1A-HRP conjugate. The assay sample and buffer are incubated together with CPT1A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CPT1A concentration since CPT1A from samples and CPT1A-HRP conjugate compete for the anti-CPT1A antibody binding site. Since the number of sites is limited, as more sites are occupied by CPT1A from the sample, fewer sites are left to bind CPT1A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CPT1A concentration in each sample is interpolated from this standard curve.
Human Carnitine O-palmitoyltransferase 1, liver isoform Protein information
Uniprot ID CPT1A_HUMAN
Uniprot AC P50416; Q8TCU0; Q9BWK0;
UniGene Hs.503043;
GeneID 1374
KEGG hsa:1374;
Human Carnitine O-palmitoyltransferase 1, liver isoform Protein SEQUENCE
SEQUENCE 773 AA; 88368 MW; E5DC9141B6301947 CRC64;
MAEAHQAVAF QFTVTPDGID LRLSHEALRQ IYLSGLHSWK KKFIRFKNGI
ITGVYPASPS SWLIVVVGVM TTMYAKIDPS LGIIAKINRT LETANCMSSQ
TKNVVSGVLF GTGLWVALIV TMRYSLKVLL SYHGWMFTEH GKMSRATKIW
MGMVKIFSGR KPMLYSFQTS LPRLPVPAVK DTVNRYLQSV RPLMKEEDFK
RMTALAQDFA VGLGPRLQWY LKLKSWWATN YVSDWWEEYI YLRGRGPLMV
NSNYYAMDLL YILPTHIQAA RAGNAIHAIL LYRRKLDREE IKPIRLLGST
IPLCSAQWER MFNTSRIPGE ETDTIQHMRD SKHIVVYHRG RYFKVWLYHD
GRLLKPREME QQMQRILDNT SEPQPGEARL AALTAGDRVP WARCRQAYFG
RGKNKQSLDA VEKAAFFVTL DETEEGYRSE DPDTSMDSYA KSLLHGRCYD
RWFDKSFTFV VFKNGKMGLN AEHSWADAPI VAHLWEYVMS IDSLQLGYAE
DGHCKGDINP NIPYPTRLQW DIPGECQEVI ETSLNTANLL ANDVDFHSFP
FVAFGKGIIK KCRTSPDAFV QLALQLAHYK DMGKFCLTYE ASMTRLFREG
RTETVRSCTT ESCDFVRAMV DPAQTVEQRL KLFKLASEKH QHMYRLAMTG
SGIDRHLFCL YVVSKYLAVE SPFLKEVLSE PWRLSTSQTP QQQVELFDLE
NNPEYVSSGG GFGPVADDGY GVSYILVGEN LINFHISSKF SCPETDSHRF
GRHLKEAMTD IITLFGLSSN SKK
UCSC uc001oof.5; human. [P50416-1];

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Human CPT1A ELISA Kit