Human CPT1A ELISA Kit

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  • Alternative name

    Human CPT1-L ELISA Kit;Human carnitine O-palmitoyltransferase I, liver isoform ELISA Kit;Human CPT I ELISA Kit;Human CPTI-L ELISA Kit;Human carnitine palmitoyltransferase 1A ELISA Kit;Human CPT1 ELISA Kit;Human L-CPT1 ELISA Kit;Human carnitine O-palmitoyltransferase 1, liver isoform ELISA Kit;Human carnitine palmitoyltransferase 1A (liver) ELISA Kit;Human carnitine palmitoyltransferase I, liver ELISA Kit;

  • Catalog
    E006734
  • species
    Human
  • GeneCPT1A
  • Other Species Mouse Cpt1a ELISA KitHorse CPT1A ELISA KitRat Cpt1a ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of CPT1A. No significant cross-reactivity or interference between CPT1A and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CPT1A and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman CPT1A ELISA Kit allows for the in vitro quantitative determination of CPT1A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Intended Uses||This CPT1A ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CPT1A. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: CPT1A ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-CPT1A antibody and an CPT1A-HRP conjugate. The assay sample and buffer are incubated together with CPT1A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CPT1A concentration since CPT1A from samples and CPT1A-HRP conjugate compete for the anti-CPT1A antibody binding site. Since the number of sites is limited, as more sites are occupied by CPT1A from the sample, fewer sites are left to bind CPT1A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CPT1A concentration in each sample is interpolated from this standard curve.
  • Human Carnitine O-palmitoyltransferase 1, liver isoform Protein information
  • Uniprot ID CPT1A_HUMAN
  • Uniprot AC P50416; Q8TCU0; Q9BWK0;
  • UniGene Hs.503043;
  • GeneID 1374
  • KEGG hsa:1374;
  • Human Carnitine O-palmitoyltransferase 1, liver isoform Protein SEQUENCE
  • SEQUENCE 773 AA; 88368 MW; E5DC9141B6301947 CRC64;

    MAEAHQAVAF QFTVTPDGID LRLSHEALRQ IYLSGLHSWK KKFIRFKNGI

    ITGVYPASPS SWLIVVVGVM TTMYAKIDPS LGIIAKINRT LETANCMSSQ

    TKNVVSGVLF GTGLWVALIV TMRYSLKVLL SYHGWMFTEH GKMSRATKIW

    MGMVKIFSGR KPMLYSFQTS LPRLPVPAVK DTVNRYLQSV RPLMKEEDFK

    RMTALAQDFA VGLGPRLQWY LKLKSWWATN YVSDWWEEYI YLRGRGPLMV

    NSNYYAMDLL YILPTHIQAA RAGNAIHAIL LYRRKLDREE IKPIRLLGST

    IPLCSAQWER MFNTSRIPGE ETDTIQHMRD SKHIVVYHRG RYFKVWLYHD

    GRLLKPREME QQMQRILDNT SEPQPGEARL AALTAGDRVP WARCRQAYFG

    RGKNKQSLDA VEKAAFFVTL DETEEGYRSE DPDTSMDSYA KSLLHGRCYD

    RWFDKSFTFV VFKNGKMGLN AEHSWADAPI VAHLWEYVMS IDSLQLGYAE

    DGHCKGDINP NIPYPTRLQW DIPGECQEVI ETSLNTANLL ANDVDFHSFP

    FVAFGKGIIK KCRTSPDAFV QLALQLAHYK DMGKFCLTYE ASMTRLFREG

    RTETVRSCTT ESCDFVRAMV DPAQTVEQRL KLFKLASEKH QHMYRLAMTG

    SGIDRHLFCL YVVSKYLAVE SPFLKEVLSE PWRLSTSQTP QQQVELFDLE

    NNPEYVSSGG GFGPVADDGY GVSYILVGEN LINFHISSKF SCPETDSHRF

    GRHLKEAMTD IITLFGLSSN SKK

  • UCSC uc001oof.5; human. [P50416-1];


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