Human CRAT ELISA Kit

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  • Alternative name

    Human carnitine acetylase ELISA Kit;Human Carnitine acetyltransferase ELISA Kit;Human CAT ELISA Kit;Human CrAT ELISA Kit;Human CAT1 ELISA Kit;Human carnitine O-acetyltransferase ELISA Kit;

  • Catalog
    E006729
  • species
    Human
  • GeneCRAT
  • Standard CurveHuman CRAT ELISA Kit
  • Other Species Mouse Crat ELISA KitRat Crat ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Carnitine Acetyltransferase (CRAT). No significant cross-reactivity or interference between Carnitine Acetyltransferase (CRAT) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 1.31ng/mL.
  • Intended UseHuman CRAT ELISA Kit allows for the in vitro quantitative determination of CRAT , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carnitine Acetyltransferase (CRAT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Carnitine Acetyltransferase (CRAT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Carnitine Acetyltransferase (CRAT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Carnitine Acetyltransferase (CRAT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Carnitine O-acetyltransferase Protein information
  • Uniprot ID CACP_HUMAN
  • Uniprot AC P43155; Q5T952; Q9BW16;
  • UniGene Hs.12068;
  • GeneID 1384
  • KEGG hsa:1384;
  • Human Carnitine O-acetyltransferase Protein SEQUENCE
  • SEQUENCE 626 AA; 70858 MW; 51B65E7C94E458D7 CRC64;

    MLAFAARTVV KPLGFLKPFS LMKASSRFKA HQDALPRLPV PPLQQSLDHY

    LKALQPIVSE EEWAHTKQLV DEFQASGGVG ERLQKGLERR ARKTENWLSE

    WWLKTAYLQY RQPVVIYSSP GVMLPKQDFV DLQGQLRFAA KLIEGVLDFK

    VMIDNETLPV EYLGGKPLCM NQYYQILSSC RVPGPKQDTV SNFSKTKKPP

    THITVVHNYQ FFELDVYHSD GTPLTADQIF VQLEKIWNSS LQTNKEPVGI

    LTSNHRNSWA KAYNTLIKDK VNRDSVRSIQ KSIFTVCLDA TMPRVSEDVY

    RSHVAGQMLH GGGSRLNSGN RWFDKTLQFI VAEDGSCGLV YEHAAAEGPP

    IVTLLDYVIE YTKKPELVRS PLVPLPMPKK LRFNITPEIK SDIEKAKQNL

    SIMIQDLDIT VMVFHHFGKD FPKSEKLSPD AFIQMALQLA YYRIYGQACA

    TYESASLRMF HLGRTDTIRS ASMDSLTFVK AMDDSSVTEH QKVELLRKAV

    QAHRGYTDRA IRGEAFDRHL LGLKLQAIED LVSMPDIFMD TSYAIAMHFH

    LSTSQVPAKT DCVMFFGPVV PDGYGVCYNP MEAHINFSLS AYNSCAETNA

    ARLAHYLEKA LLDMRALLQS HPRAKL

  • UCSC uc004bxh.4; human. [P43155-1];


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