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Alternative name
Selenoprotein S ELISA KIT; Minor histocompatibility antigen H47 ELISA KIT; VCP-interacting membrane proteinVimp ELISA KIT; H47 ELISA KIT; Sels ELISA KIT; SelS ELISA KIT
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Catalog
E067234
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species
Mouse
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GeneSels
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Standard Curve
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Other Species
Human SELS ELISA Kit
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SpecificityThis assay recognizes recombinant and natural mouse SelS. No significant cross-reactivity or interference was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Intended UseMouse Sels ELISA Kit allows for the in vitro quantitative determination of Sels , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to SelS. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for SelS and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain SelS, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of SelS in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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