SpecificityThis assay has high sensitivity and excellent specificity for detection of SFRP. No significant cross-reactivity or interference between SFRP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between SFRP and all the analogues, therefore, cross reaction may still exist in some cases.
Intended UseMouse SFRP ELISA Kit allows for the in vitro quantitative determination of SFRP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Categories/FamilyCell Biology
Product Description specificalIntended Uses: This SFRP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse SFRP. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Principle of the Assay||SFRP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SFRP antibody and an SFRP-HRP conjugate. The assay sample and buffer are incubated together with SFRP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SFRP concentration since SFRP from samples and SFRP-HRP conjugate compete for the anti-SFRP antibody binding site. Since the number of sites is limited, as more sites are occupied by SFRP from the sample, fewer sites are left to bind SFRP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SFRP concentration in each sample is interpolated from this standard curve.