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Alternative name
Mouse S-100 protein beta chain ELISA Kit;Mouse S-100 protein subunit beta ELISA Kit;Mouse S100 calcium-binding protein B ELISA Kit;Mouse AI850290 ELISA Kit;Mouse Bpb ELISA Kit;Mouse S100 protein, beta polypeptide, neural ELISA Kit;Mouse protein S100-B ELISA Kit;
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Catalog
E066921
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species
Mouse
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GeneS100b
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity<46.9pg/ml
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Intended UseMouse S100b ELISA Kit allows for the in vitro quantitative determination of S100b , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageStore the whole ELISA kit at 4℃
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Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Mouse S100B antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse S100B antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse S100B amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse S100B can be calculated.
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Mouse Protein S100-B Protein information
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Uniprot ID
S100B_MOUSE
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Uniprot AC
P50114;
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UniGene
Mm.235998;
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GeneID
20203
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KEGG
mmu:20203;
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Mouse Protein S100-B Protein SEQUENCE
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SEQUENCE 92 AA; 10728 MW; 43815AC212A81DD0 CRC64;
MSELEKAMVA LIDVFHQYSG REGDKHKLKK SELKELINNE LSHFLEEIKE
QEVVDKVMET
LDEDGDGECD FQEFMAFVAM VTTACHEFFE HE
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UCSC
uc007fuc.2; mouse.;
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