Mouse S100A7 ELISA Kit

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  • Alternative name

    Protein S100-A7 ELISA KIT; Psoriasin ELISA KIT; S100 calcium-binding protein A7S100A7 ELISA KIT; PSOR1 ELISA KIT; S100A7C ELISA KIT

  • Catalog
    E066908
  • species
    Mouse
  • GeneS100A7
  • Standard CurveMouse S100A7 ELISA Kit
  • Other Species Human S100A7 ELISA KitBovine S100A7 ELISA KitHorse s100A7 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 pg/mL.
  • Intended UseMouse S100A7 ELISA Kit allows for the in vitro quantitative determination of S100A7 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical
    Intended Uses: This S100A7 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse S100A7. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: S100A7 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for S100A7. Standards or samples are then added to the microtiter plate wells and S100A7 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of S100A7 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for S100A7 are added to each well to "sandwich" the S100A7 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain S100A7 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The S100A7 concentration in each sample is interpolated from this standard curve.


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