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Alternative name
Protein S100-A12 ELISA KIT; CGRP ELISA KIT; Calcium-binding protein in amniotic fluid 1 ELISA KIT; CAAF1 ELISA KIT; Calgranulin-C ELISA KIT; CAGC ELISA KIT; Extracellular newly identified RAGE-binding protein ELISA KIT; EN-RAGE ELISA KIT; Migration inhibitory factor-related protein 6 ELISA KIT; MRP-6 ELISA KIT; p6 ELISA KIT; Neutrophil S100 protein ELISA KIT; S100 calcium-binding protein A12Cleaved into the following chain:CalciterminS100A12 ELISA KIT; CAAF1 ELISA KIT; CAGC ELISA KIT; EN-RAGE ELISA KIT; MRP-6 ELISA KIT; p6 ELISA KIT
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Catalog
E066888
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species
Mouse
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GeneS100A12
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SpecificityThis assay has high sensitivity and excellent specificity for detection of S100A12. No significant cross-reactivity or interference between S100A12 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between S100A12 and all the analogues, therefore, cross reaction may still exist in some cases.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity1.0 pg/mL.
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Intended UseMouse S100A12 ELISA Kit allows for the in vitro quantitative determination of S100A12 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Categories/FamilySignal Transduction
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Product Description
specificalIntended Uses: This S100A12 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse S100A12. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
Principle of the Assay||S100A12 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for S100A12. Standards or samples are then added to the microtiter plate wells and S100A12 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of S100A12 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for S100A12 are added to each well to "sandwich" the S100A12 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain S100A12 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The S100A12 concentration in each sample is interpolated from this standard curve.
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