Mouse Sag ELISA Kit

Alternative name
Mouse 48 kDa protein ELISA Kit;Mouse retinal S-antigen ELISA Kit;Mouse S-AG ELISA Kit;Mouse rod photoreceptor arrestin ELISA Kit;Mouse A930001K18Rik ELISA Kit;Mouse Arr1 ELISA Kit;Mouse Irbp ELISA Kit;Mouse arrestin ELISA Kit;Mouse S-antigen, retina and pineal gland (arrestin) ELISA Kit;Mouse S-arrestin ELISA Kit;Mouse arrestin 1 ELISA Kit;Mouse rod arrestin ELISA Kit;Mouse visual arrestin 1 ELISA Kit;
Catalog
E066853
species
Mouse
GeneSag
Other Species Human HCV-SAG ELISA Kit Human SAG ELISA Kit Mouse HCV-SAG ELISA Kit Bovine SAG ELISA Kit Canine SAG ELISA Kit Porcine SAG ELISA Kit Rat Sag ELISA Kit
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseMouse Sag ELISA Kit allows for the in vitro quantitative determination of Sag , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyImmunology
Product Description
specificalPrinciple of the Assay: SAG ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-SAG antibody and an SAG-HRP conjugate. The assay sample and buffer are incubated together with SAG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the SAG concentration since SAG from samples and SAG-HRP conjugate compete for the anti-SAG antibody binding site. Since the number of sites is limited, as more sites are occupied by SAG from the sample, fewer sites are left to bind SAG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The SAG concentration in each sample is interpolated from this standard curve.
Mouse S-arrestin Protein information
Uniprot ID ARRS_MOUSE
Uniprot AC P20443; Q91W62;
UniGene Mm.1276;
GeneID 20215
KEGG mmu:20215;
Mouse S-arrestin Protein SEQUENCE
SEQUENCE 403 AA; 44930 MW; 2D4CFEAF81C5FC6D CRC64;
MAACGKTNKS HVIFKKVSRD KSVTIYLGKR DYVDHVSQVE PVDGVVLVDP
ELVKGKKVYV TLTCAFRYGQ EDIDVMGLTF RRDLYFSRVQ VYPPVGAMSV
LTQLQESLLK KLGDNTYPFL LTFPDYLPCS VMLQPAPQDV GKSCGVDFEV
KAFASDITDP EEDKIPKKSS VRLLIRKVQH APPEMGPQPS AEASWQFFMS
DKPLNLSVSL SKEIYFHGEP IPVTVTVTNN TDKVVKKIKV SVEQIANVVL
YSSDYYVKPV ASEETQEKVQ PNSTLTKTLV LVPLLANNRE RRGIALDGKI
KHEDTNLASS TIIKEGIDRT VMGILVSYHI KVKLTVSGFL GELTSSEVAT
EVPFRLMHPQ PEDPAKESVQ DENLVFEEFA RQNLKDTGEN TEGKKDEDAG
QDE
UCSC uc007bxp.1; mouse.;

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Mouse Sag ELISA Kit