Mouse Robo1 ELISA Kit

Alternative name
Mouse DUTT1 ELISA Kit;Mouse AW494633 ELISA Kit;Mouse AW742721 ELISA Kit;Mouse Gm310 ELISA Kit;Mouse roundabout guidance receptor 1 ELISA Kit;Mouse roundabout homolog 1 ELISA Kit;
Catalog
E066793
species
Mouse
GeneRobo1
SpecificityThis assay recognizes recombinant and natural mouse Roundabout homolog 1. No significant cross-reactivity or interference was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Intended UseMouse Robo1 ELISA Kit allows for the in vitro quantitative determination of Robo1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Roundabout homolog 1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Roundabout homolog 1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Roundabout homolog 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Roundabout homolog 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Mouse Roundabout homolog 1 Protein information
Uniprot ID ROBO1_MOUSE
Uniprot AC O89026;
UniGene Mm.310772;
GeneID 19876
KEGG mmu:19876;
Mouse Roundabout homolog 1 Protein SEQUENCE
SEQUENCE 1612 AA; 176407 MW; 5F2988C544796B4B CRC64;
MIAEPAHFYL FGLICLCSGS RLRQEDFPPR IVEHPSDLIV SKGEPATLNC
KAEGRPTPTI EWYKGGERVE TDKDDPRSHR MLLPSGSLFF LRIVHGRKSR
PDEGVYICVA RNYLGEAVSH NASLEVAILR DDFRQNPSDV MVAVGEPAVM
ECQPPRGHPE PTISWKKDGS PLDDKDERIT IRGGKLMITY TRKSDAGKYV
CVGTNMVGER ESEVAELTVL ERPSFVKRPS NLAVTVDDSA EFKCEARGDP
VPTVRWRKDD GELPKSRYEI RDDHTLKIRK VTAGDMGSYT CVAENMVGKA
EASATLTVQE PPHFVVKPRD QVVALGRTVT FQCEATGNPQ PAIFWRREGS
QNLLFSYQPP QSSSRFSVSQ TGDLTITNVQ RSDVGYYICQ TLNVAGSIIT
KAYLEVTDVI ADRPPPVIRQ GPVNQTVAVD GTLILSCVAT GSPAPTILWR
KDGVLVSTQD SRIKQLESGV LQIRYAKLGD TGRYTCTAST PSGEATWSAY
IEVQEFGVPV QPPRPTDPNL IPSAPSKPEV TDVSKNTVTL SWQPNLNSGA
TPTSYIIEAF SHASGSSWQT AAENVKTETF AIKGLKPNAI YLFLVRAANA
YGISDPSQIS DPVKTQDVPP TSQGVDHKQV QRELGNVVLH LHNPTILSSS
SVEVHWTVDQ QSQYIQGYKI LYRPSGASHG ESEWLVFEVR TPTKNSVVIP
DLRKGVNYEI KARPFFNEFQ GADSEIKFAK TLEEAPSAPP RSVTVSKNDG
NGTAILVTWQ PPPEDTQNGM VQEYKVWCLG NETKYHINKT VDGSTFSVVI
PSLVPGIRYS VEVAASTGAG PGVKSEPQFI QLDSHGNPVS PEDQVSLAQQ
ISDVVRQPAF IAGIGAACWI ILMVFSIWLY RHRKKRNGLT STYAGIRKVP
SFTFTPTVTY QRGGEAVSSG GRPGLLNISE PATQPWLADT WPNTGNNHND
CSINCCTAGN GNSDSNLTTY SRPADCIANY NNQLDNKQTN LMLPESTVYG
DVDLSNKINE MKTFNSPNLK DGRFVNPSGQ PTPYATTQLI QANLSNNMNN
GAGDSSEKHW KPPGQQKPEV APIQYNIMEQ NKLNKDYRAN DTIPPTIPYN
QSYDQNTGGS YNSSDRGSST SGSQGHKKGA RTPKAPKQGG MNWADLLPPP
PAHPPPHSNS EEYNMSVDES YDQEMPCPVP PAPMYLQQDE LQEEEDERGP
TPPVRGAASS PAAVSYSHQS TATLTPSPQE ELQPMLQDCP EDLGHMPHPP
DRRRQPVSPP PPPRPISPPH TYGYISGPLV SDMDTDAPEE EEDEADMEVA
KMQTRRLLLR GLEQTPASSV GDLESSVTGS MINGWGSASE EDNISSGRSS
VSSSDGSFFT DADFAQAVAA AAEYAGLKVA RRQMQDAAGR RHFHASQCPR
PTSPVSTDSN MSAVVIQKAR PAKKQKHQPG HLRREAYADD LPPPPVPPPA
IKSPTVQSKA QLEVRPVMVP KLASIEARTD RSSDRKGGSY KGREALDGRQ
VTDLRTNPSD PREAQEQPND GKGRGTRQPK RDLPPAKTHL GQEDILPYCR
PTFPTSNNPR DPSSSSSMSS RGSGSRQREQ ANVGRRNMAE MQVLGGFERG
DENNEELEET ES

Category

Mouse Robo1 ELISA Kit

Mouse DUTT1 ELISA Kit;Mouse AW494633 ELISA Kit;Mouse AW742721 ELISA Kit;Mouse Gm310 ELISA Kit;Mouse roundabout guidance receptor 1 ELISA Kit;Mouse roundabout homolog 1 ELISA Kit;