Mouse Robo1 ELISA Kit

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  • Alternative name

    Mouse DUTT1 ELISA Kit;Mouse AW494633 ELISA Kit;Mouse AW742721 ELISA Kit;Mouse Gm310 ELISA Kit;Mouse roundabout guidance receptor 1 ELISA Kit;Mouse roundabout homolog 1 ELISA Kit;

  • Catalog
    E066793
  • species
    Mouse
  • GeneRobo1
  • Standard CurveMouse Robo1 ELISA Kit
  • Other Species Human ROBO1 ELISA KitRat Robo1 ELISA Kit
  • SpecificityThis assay recognizes recombinant and natural mouse Roundabout homolog 1. No significant cross-reactivity or interference was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseMouse Robo1 ELISA Kit allows for the in vitro quantitative determination of Robo1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Roundabout homolog 1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for Roundabout homolog 1 and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain Roundabout homolog 1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Roundabout homolog 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Mouse Roundabout homolog 1 Protein information
  • Uniprot ID ROBO1_MOUSE
  • Uniprot AC O89026;
  • UniGene Mm.310772;
  • GeneID 19876
  • KEGG mmu:19876;
  • Mouse Roundabout homolog 1 Protein SEQUENCE
  • SEQUENCE 1612 AA; 176407 MW; 5F2988C544796B4B CRC64;

    MIAEPAHFYL FGLICLCSGS RLRQEDFPPR IVEHPSDLIV SKGEPATLNC

    KAEGRPTPTI EWYKGGERVE TDKDDPRSHR MLLPSGSLFF LRIVHGRKSR

    PDEGVYICVA RNYLGEAVSH NASLEVAILR DDFRQNPSDV MVAVGEPAVM

    ECQPPRGHPE PTISWKKDGS PLDDKDERIT IRGGKLMITY TRKSDAGKYV

    CVGTNMVGER ESEVAELTVL ERPSFVKRPS NLAVTVDDSA EFKCEARGDP

    VPTVRWRKDD GELPKSRYEI RDDHTLKIRK VTAGDMGSYT CVAENMVGKA

    EASATLTVQE PPHFVVKPRD QVVALGRTVT FQCEATGNPQ PAIFWRREGS

    QNLLFSYQPP QSSSRFSVSQ TGDLTITNVQ RSDVGYYICQ TLNVAGSIIT

    KAYLEVTDVI ADRPPPVIRQ GPVNQTVAVD GTLILSCVAT GSPAPTILWR

    KDGVLVSTQD SRIKQLESGV LQIRYAKLGD TGRYTCTAST PSGEATWSAY

    IEVQEFGVPV QPPRPTDPNL IPSAPSKPEV TDVSKNTVTL SWQPNLNSGA

    TPTSYIIEAF SHASGSSWQT AAENVKTETF AIKGLKPNAI YLFLVRAANA

    YGISDPSQIS DPVKTQDVPP TSQGVDHKQV QRELGNVVLH LHNPTILSSS

    SVEVHWTVDQ QSQYIQGYKI LYRPSGASHG ESEWLVFEVR TPTKNSVVIP

    DLRKGVNYEI KARPFFNEFQ GADSEIKFAK TLEEAPSAPP RSVTVSKNDG

    NGTAILVTWQ PPPEDTQNGM VQEYKVWCLG NETKYHINKT VDGSTFSVVI

    PSLVPGIRYS VEVAASTGAG PGVKSEPQFI QLDSHGNPVS PEDQVSLAQQ

    ISDVVRQPAF IAGIGAACWI ILMVFSIWLY RHRKKRNGLT STYAGIRKVP

    SFTFTPTVTY QRGGEAVSSG GRPGLLNISE PATQPWLADT WPNTGNNHND

    CSINCCTAGN GNSDSNLTTY SRPADCIANY NNQLDNKQTN LMLPESTVYG

    DVDLSNKINE MKTFNSPNLK DGRFVNPSGQ PTPYATTQLI QANLSNNMNN

    GAGDSSEKHW KPPGQQKPEV APIQYNIMEQ NKLNKDYRAN DTIPPTIPYN

    QSYDQNTGGS YNSSDRGSST SGSQGHKKGA RTPKAPKQGG MNWADLLPPP

    PAHPPPHSNS EEYNMSVDES YDQEMPCPVP PAPMYLQQDE LQEEEDERGP

    TPPVRGAASS PAAVSYSHQS TATLTPSPQE ELQPMLQDCP EDLGHMPHPP

    DRRRQPVSPP PPPRPISPPH TYGYISGPLV SDMDTDAPEE EEDEADMEVA

    KMQTRRLLLR GLEQTPASSV GDLESSVTGS MINGWGSASE EDNISSGRSS

    VSSSDGSFFT DADFAQAVAA AAEYAGLKVA RRQMQDAAGR RHFHASQCPR

    PTSPVSTDSN MSAVVIQKAR PAKKQKHQPG HLRREAYADD LPPPPVPPPA

    IKSPTVQSKA QLEVRPVMVP KLASIEARTD RSSDRKGGSY KGREALDGRQ

    VTDLRTNPSD PREAQEQPND GKGRGTRQPK RDLPPAKTHL GQEDILPYCR

    PTFPTSNNPR DPSSSSSMSS RGSGSRQREQ ANVGRRNMAE MQVLGGFERG

    DENNEELEET ES



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