Mouse Sbds ELISA Kit

Alternative name
Mouse protein 22A3 ELISA Kit;Mouse shwachman-Bodian-Diamond syndrome protein homolog ELISA Kit;Mouse 4733401P19Rik ELISA Kit;Mouse AI836084 ELISA Kit;Mouse CGI-97 ELISA Kit;Mouse SDS ELISA Kit;Mouse Shwachman-Bodian-Diamond syndrome homolog (human) ELISA Kit;Mouse ribosome maturation protein SBDS ELISA Kit;
Catalog
E066618
species
Mouse
GeneSbds
SpecificityThis assay has high sensitivity and excellent specificity for detection of RMP. No significant cross-reactivity or interference between RMP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between RMP and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL
Intended UseMouse Sbds ELISA Kit allows for the in vitro quantitative determination of Sbds , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalIntended Uses: This RMP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse RMP. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: RMP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-RMP antibody and an RMP-HRP conjugate. The assay sample and buffer are incubated together with RMP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RMP concentration since RMP from samples and RMP-HRP conjugate compete for the anti-RMP antibody binding site. Since the number of sites is limited, as more sites are occupied by RMP from the sample, fewer sites are left to bind RMP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RMP concentration in each sample is interpolated from this standard curve.
Mouse Ribosome maturation protein SBDS Protein information
Uniprot ID SBDS_MOUSE
Uniprot AC P70122; Q3TG78; Q9CR18;
UniGene Mm.280484;
GeneID 66711
KEGG mmu:66711;
Mouse Ribosome maturation protein SBDS Protein SEQUENCE
SEQUENCE 250 AA; 28781 MW; 3137CA2807DD250A CRC64;
MSIFTPTNQI RLTNVAVVRM KRGGKRFEIA CYKNKVVGWR SGVEKDLDEV
LQTHSVFVNV SKGQVAKKED LISAFGTDDQ TEICKQILTK GEVQVSDKER
HTQLEQMFRD IATIVADKCV NPETKRPYTV ILIERAMKDI HYSVKPNKST
KQQALEVIKQ LKEKMKIERA HMRLRFILPV NEGKKLKEKL KPLMKVVESE
DYSQQLEIVC LIDPGCFREI DELIKKETKG RGSLEVLSLK DVEEGDEKFE
UCSC uc008zuj.1; mouse.;

Mouse Sbds ELISA Kit

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