Mouse Sbds ELISA Kit

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  • Alternative name

    Mouse protein 22A3 ELISA Kit;Mouse shwachman-Bodian-Diamond syndrome protein homolog ELISA Kit;Mouse 4733401P19Rik ELISA Kit;Mouse AI836084 ELISA Kit;Mouse CGI-97 ELISA Kit;Mouse SDS ELISA Kit;Mouse Shwachman-Bodian-Diamond syndrome homolog (human) ELISA Kit;Mouse ribosome maturation protein SBDS ELISA Kit;

  • Catalog
    E066618
  • species
    Mouse
  • GeneSbds
  • Standard CurveMouse Sbds ELISA Kit
  • Other Species Human SBDS ELISA KitBovine SBDS ELISA KitChicken SBDS ELISA KitRat Sbds ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of RMP. No significant cross-reactivity or interference between RMP and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between RMP and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL
  • Intended UseMouse Sbds ELISA Kit allows for the in vitro quantitative determination of Sbds , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Intended Uses: This RMP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse RMP. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: RMP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-RMP antibody and an RMP-HRP conjugate. The assay sample and buffer are incubated together with RMP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RMP concentration since RMP from samples and RMP-HRP conjugate compete for the anti-RMP antibody binding site. Since the number of sites is limited, as more sites are occupied by RMP from the sample, fewer sites are left to bind RMP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RMP concentration in each sample is interpolated from this standard curve.
  • Mouse Ribosome maturation protein SBDS Protein information
  • Uniprot ID SBDS_MOUSE
  • Uniprot AC P70122; Q3TG78; Q9CR18;
  • UniGene Mm.280484;
  • GeneID 66711
  • KEGG mmu:66711;
  • Mouse Ribosome maturation protein SBDS Protein SEQUENCE
  • SEQUENCE 250 AA; 28781 MW; 3137CA2807DD250A CRC64;

    MSIFTPTNQI RLTNVAVVRM KRGGKRFEIA CYKNKVVGWR SGVEKDLDEV

    LQTHSVFVNV SKGQVAKKED LISAFGTDDQ TEICKQILTK GEVQVSDKER

    HTQLEQMFRD IATIVADKCV NPETKRPYTV ILIERAMKDI HYSVKPNKST

    KQQALEVIKQ LKEKMKIERA HMRLRFILPV NEGKKLKEKL KPLMKVVESE

    DYSQQLEIVC LIDPGCFREI DELIKKETKG RGSLEVLSLK DVEEGDEKFE

  • UCSC uc008zuj.1; mouse.;


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