Human CEACAM1 ELISA Kit

Alternative name
Human Biliary glycoprotein 1 ELISA Kit;Human BGP-1 ELISA Kit;Human BGP ELISA Kit;Human BGP1 ELISA Kit;Human BGPI ELISA Kit;Human carcinoembryonic antigen related cell adhesion molecule 1 ELISA Kit;Human carcinoembryonic antigen-related cell adhesion molecule 1 ELISA Kit;Human CD66a antigen ELISA Kit;Human antigen CD66 ELISA Kit;Human carcinoembryonic antigen-related cell adhesion molecule 1 (biliary glycoprotein) ELISA Kit;
Catalog
E006635
species
Human
GeneCEACAM1
Standard Curve
Other Species Mouse Ceacam1 ELISA Kit Rat Ceacam1 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of CEACAM1. No significant cross-reactivity or interference between CEACAM1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CEACAM1 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 ng/mL.
Intended UseHuman CEACAM1 ELISA Kit allows for the in vitro quantitative determination of CEACAM1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyCell Biology
Product Description
specificalPrinciple of the assay: CEACAM1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CEACAM1 antibody and an CEACAM1-HRP conjugate. The assay sample and buffer are incubated together with CEACAM1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CEACAM1 concentration since CEACAM1 from samples and CEACAM1-HRP conjugate compete for the anti-CEACAM1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CEACAM1 from the sample, fewer sites are left to bind CEACAM1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CEACAM1 concentration in each sample is interpolated from this standard curve.
Human Carcinoembryonic antigen-related cell adhesion molecule 1 Protein information
Uniprot ID CEAM1_HUMAN
Uniprot AC Q9UQV9;
UniGene Hs.512682;
GeneID 634
KEGG hsa:634;
Human Carcinoembryonic antigen-related cell adhesion molecule 1 Protein SEQUENCE
SEQUENCE 526 AA; 57560 MW; CAD1B2328D069AF8 CRC64;
MGHLSAPLHR VRVPWQGLLL TASLLTFWNP PTTAQLTTES MPFNVAEGKE
VLLLVHNLPQ QLFGYSWYKG ERVDGNRQIV GYAIGTQQAT PGPANSGRET
IYPNASLLIQ NVTQNDTGFY TLQVIKSDLV NEEATGQFHV YPELPKPSIS
SNNSNPVEDK DAVAFTCEPE TQDTTYLWWI NNQSLPVSPR LQLSNGNRTL
TLLSVTRNDT GPYECEIQNP VSANRSDPVT LNVTYGPDTP TISPSDTYYR
PGANLSLSCY AASNPPAQYS WLINGTFQQS TQELFIPNIT VNNSGSYTCH
ANNSVTGCNR TTVKTIIVTE LSPVVAKPQI KASKTTVTGD KDSVNLTCST
NDTGISIRWF FKNQSLPSSE RMKLSQGNTT LSINPVKRED AGTYWCEVFN
PISKNQSDPI MLNVNYNALP QENGLSPGAI AGIVIGVVAL VALIAVALAC
FLHFGKTGRA SDQRDLTEHK PSVSNHTQDH SNDPPNKMNE VTYSTLNFEA
QQPTQPTSAS PSLTATEIIY SEVKKQ
UCSC uc002otv.3; human. [P13688-1];

Category

Human CEACAM1 ELISA Kit