Human CPZ ELISA Kit

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  • Alternative name

    Human CPZ ELISA Kit;Human carboxypeptidase Z ELISA Kit;Human metallocarboxypeptidase Z ELISA Kit;

  • Catalog
    E006622
  • species
    Human
  • GeneCPZ
  • Standard CurveHuman CPZ ELISA Kit
  • Other Species Mouse Cpz ELISA KitChicken CPZ ELISA KitRat Cpz ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Carboxypeptidase Z (CPZ). No significant cross-reactivity or interference between Carboxypeptidase Z (CPZ) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.226ng/mL.
  • Intended UseHuman CPZ ELISA Kit allows for the in vitro quantitative determination of CPZ , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carboxypeptidase Z (CPZ). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Carboxypeptidase Z (CPZ). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Carboxypeptidase Z (CPZ), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Carboxypeptidase Z (CPZ) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Carboxypeptidase Z Protein information
  • Uniprot ID CBPZ_HUMAN
  • Uniprot AC Q66K79; O00520; Q96MX2;
  • UniGene Hs.61995;
  • GeneID 8532
  • KEGG hsa:8532;
  • Human Carboxypeptidase Z Protein SEQUENCE
  • SEQUENCE 652 AA; 73655 MW; BFA1EA7753E63F98 CRC64;

    MPPPLPLLLL TVLVVAAARP GCEFERNPAG ECHRPPAADS ATCVDLQLRT

    CSDAAYNHTT FPNLLQHRSW EVVEASSEYI LLSVLHQLLE GQCNPDLRLL

    GCAVLAPRCE GGWVRRPCRH ICEGLREVCQ PAFDAIDMAW PYFLDCHRYF

    TREDEGCYDP LEKLRGGLEA DEALPSGLPP TFIRFSHHSY AQMVRVLRRT

    ASRCAHVART YSIGRSFDGR ELLVIEFSSR PGQHELMEPE VKLIGNIHGN

    EVAGREMLIY LAQYLCSEYL LGNPRIQRLL NTTRIHLLPS MNPDGYEVAA

    AEGAGYNGWT SGRQNAQNLD LNRNFPDLTS EYYRLAETRG ARSDHIPIPQ

    HYWWGKVAPE TKAIMKWMQT IPFVLSASLH GGDLVVSYPF DFSKHPQEEK

    MFSPTPDEKM FKLLSRAYAD VHPMMMDRSE NRCGGNFLKR GSIINGADWY

    SFTGGMSDFN YLHTNCFEIT VELGCVKFPP EEALYILWQH NKESLLNFVE

    TVHRGIKGVV TDKFGKPVKN ARISVKGIRH DITTAPDGDY WRLLPPGIHI

    VIAQAPGYAK VIKKVIIPAR MKRAGRVDFI LQPLGMGPKN FIHGLRRTGP

    HDPLGGASSL GEATEPDPLR ARRQPSADGS KPWWWSYFTS LSTHRPRWLL

    KY

  • UCSC uc003glm.4; human. [Q66K79-1];


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