Human CPZ ELISA Kit

Alternative name
Human CPZ ELISA Kit;Human carboxypeptidase Z ELISA Kit;Human metallocarboxypeptidase Z ELISA Kit;
Catalog
E006622
species
Human
GeneCPZ
SpecificityThis assay has high sensitivity and excellent specificity for detection of Carboxypeptidase Z (CPZ). No significant cross-reactivity or interference between Carboxypeptidase Z (CPZ) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.226ng/mL.
Intended UseHuman CPZ ELISA Kit allows for the in vitro quantitative determination of CPZ , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carboxypeptidase Z (CPZ). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Carboxypeptidase Z (CPZ). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Carboxypeptidase Z (CPZ), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Carboxypeptidase Z (CPZ) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Carboxypeptidase Z Protein information
Uniprot ID CBPZ_HUMAN
Uniprot AC Q66K79; O00520; Q96MX2;
UniGene Hs.61995;
GeneID 8532
KEGG hsa:8532;
Human Carboxypeptidase Z Protein SEQUENCE
SEQUENCE 652 AA; 73655 MW; BFA1EA7753E63F98 CRC64;
MPPPLPLLLL TVLVVAAARP GCEFERNPAG ECHRPPAADS ATCVDLQLRT
CSDAAYNHTT FPNLLQHRSW EVVEASSEYI LLSVLHQLLE GQCNPDLRLL
GCAVLAPRCE GGWVRRPCRH ICEGLREVCQ PAFDAIDMAW PYFLDCHRYF
TREDEGCYDP LEKLRGGLEA DEALPSGLPP TFIRFSHHSY AQMVRVLRRT
ASRCAHVART YSIGRSFDGR ELLVIEFSSR PGQHELMEPE VKLIGNIHGN
EVAGREMLIY LAQYLCSEYL LGNPRIQRLL NTTRIHLLPS MNPDGYEVAA
AEGAGYNGWT SGRQNAQNLD LNRNFPDLTS EYYRLAETRG ARSDHIPIPQ
HYWWGKVAPE TKAIMKWMQT IPFVLSASLH GGDLVVSYPF DFSKHPQEEK
MFSPTPDEKM FKLLSRAYAD VHPMMMDRSE NRCGGNFLKR GSIINGADWY
SFTGGMSDFN YLHTNCFEIT VELGCVKFPP EEALYILWQH NKESLLNFVE
TVHRGIKGVV TDKFGKPVKN ARISVKGIRH DITTAPDGDY WRLLPPGIHI
VIAQAPGYAK VIKKVIIPAR MKRAGRVDFI LQPLGMGPKN FIHGLRRTGP
HDPLGGASSL GEATEPDPLR ARRQPSADGS KPWWWSYFTS LSTHRPRWLL
KY
UCSC uc003glm.4; human. [Q66K79-1];

Category

Human CPZ ELISA Kit

Human CPZ ELISA Kit;Human carboxypeptidase Z ELISA Kit;Human metallocarboxypeptidase Z ELISA Kit;