Human CPM ELISA Kit

Alternative name
Human CPM ELISA Kit;Human carboxypeptidase M ELISA Kit;Human renal carboxypeptidase ELISA Kit;Human urinary carboxypeptidase B ELISA Kit;
Catalog
E006604
species
Human
GeneCPM
Standard Curve
Other Species Mouse Cpm ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of Carboxypeptidase M (CPM). No significant cross-reactivity or interference between Carboxypeptidase M (CPM) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 28pg/mL.
Intended UseHuman CPM ELISA Kit allows for the in vitro quantitative determination of CPM , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carboxypeptidase M (CPM). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Carboxypeptidase M (CPM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Carboxypeptidase M (CPM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Carboxypeptidase M (CPM) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Carboxypeptidase M Protein information
Uniprot ID CBPM_HUMAN
Uniprot AC P14384; B2R800; Q9H2K9;
UniGene Hs.654387;
GeneID 1368
KEGG hsa:1368;
Human Carboxypeptidase M Protein SEQUENCE
SEQUENCE 443 AA; 50514 MW; 98EB0C94E201B901 CRC64;
MDFPCLWLGL LLPLVAALDF NYHRQEGMEA FLKTVAQNYS SVTHLHSIGK
SVKGRNLWVL VVGRFPKEHR IGIPEFKYVA NMHGDETVGR ELLLHLIDYL
VTSDGKDPEI TNLINSTRIH IMPSMNPDGF EAVKKPDCYY SIGRENYNQY
DLNRNFPDAF EYNNVSRQPE TVAVMKWLKT ETFVLSANLH GGALVASYPF
DNGVQATGAL YSRSLTPDDD VFQYLAHTYA SRNPNMKKGD ECKNKMNFPN
GVTNGYSWYP LQGGMQDYNY IWAQCFEITL ELSCCKYPRE EKLPSFWNNN
KASLIEYIKQ VHLGVKGQVF DQNGNPLPNV IVEVQDRKHI CPYRTNKYGE
YYLLLLPGSY IINVTVPGHD PHITKVIIPE KSQNFSALKK DILLPFQGQL
DSIPVSNPSC PMIPLYRNLP DHSAATKPSL FLFLVSLLHI FFK
UCSC uc001sup.4; human.;

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anti-Carboxypeptidase M antibody

Category

Human CPM ELISA Kit

Human CPM ELISA Kit;Human carboxypeptidase M ELISA Kit;Human renal carboxypeptidase ELISA Kit;Human urinary carboxypeptidase B ELISA Kit;