Human CPM ELISA Kit

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  • Alternative name

    Human CPM ELISA Kit;Human carboxypeptidase M ELISA Kit;Human renal carboxypeptidase ELISA Kit;Human urinary carboxypeptidase B ELISA Kit;

  • Catalog
    E006604
  • species
    Human
  • GeneCPM
  • Standard CurveHuman CPM ELISA Kit
  • Other Species Mouse Cpm ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Carboxypeptidase M (CPM). No significant cross-reactivity or interference between Carboxypeptidase M (CPM) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 28pg/mL.
  • Intended UseHuman CPM ELISA Kit allows for the in vitro quantitative determination of CPM , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carboxypeptidase M (CPM). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Carboxypeptidase M (CPM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Carboxypeptidase M (CPM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Carboxypeptidase M (CPM) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Carboxypeptidase M Protein information
  • Uniprot ID CBPM_HUMAN
  • Uniprot AC P14384; B2R800; Q9H2K9;
  • UniGene Hs.654387;
  • GeneID 1368
  • KEGG hsa:1368;
  • Human Carboxypeptidase M Protein SEQUENCE
  • SEQUENCE 443 AA; 50514 MW; 98EB0C94E201B901 CRC64;

    MDFPCLWLGL LLPLVAALDF NYHRQEGMEA FLKTVAQNYS SVTHLHSIGK

    SVKGRNLWVL VVGRFPKEHR IGIPEFKYVA NMHGDETVGR ELLLHLIDYL

    VTSDGKDPEI TNLINSTRIH IMPSMNPDGF EAVKKPDCYY SIGRENYNQY

    DLNRNFPDAF EYNNVSRQPE TVAVMKWLKT ETFVLSANLH GGALVASYPF

    DNGVQATGAL YSRSLTPDDD VFQYLAHTYA SRNPNMKKGD ECKNKMNFPN

    GVTNGYSWYP LQGGMQDYNY IWAQCFEITL ELSCCKYPRE EKLPSFWNNN

    KASLIEYIKQ VHLGVKGQVF DQNGNPLPNV IVEVQDRKHI CPYRTNKYGE

    YYLLLLPGSY IINVTVPGHD PHITKVIIPE KSQNFSALKK DILLPFQGQL

    DSIPVSNPSC PMIPLYRNLP DHSAATKPSL FLFLVSLLHI FFK

  • UCSC uc001sup.4; human.;


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