Mouse Rapgef2 ELISA Kit

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  • Alternative name

    Mouse cyclic nucleotide ras GEF ELISA Kit;Mouse CNRasGEF ELISA Kit;Mouse neural RAP guanine nucleotide exchange protein ELISA Kit;Mouse nRap GEP ELISA Kit;Mouse PDZ domain-containing guanine nucleotide exchange factor 1 ELISA Kit;Mouse PDZ-GEF1 ELISA Kit;Mouse RA-GEF-1 ELISA Kit;Mouse ras/Rap1-associating GEF-1 ELISA Kit;Mouse Kiaa0313 ELISA Kit;Mouse Pdzgef1 ELISA Kit;Mouse 5830453M24Rik ELISA Kit;Mouse mKIAA0313 ELISA Kit;Mouse nRapGEP ELISA Kit;Mouse Rap guanine nucleotide exchange factor (GEF) 2 ELISA Kit;Mouse rap guanine nucleotide exchange factor 2 ELISA Kit;

  • Catalog
    E065892
  • species
    Mouse
  • GeneRapgef2
  • Other Species Bovine RAPGEF2 ELISA KitCanine RAPGEF2 ELISA KitHuman RAPGEF2 ELISA KitRat Rapgef2 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse RAPGEF2. No significant cross-reactivity or interference between Mouse RAPGEF2 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse RAPGEF2 and all the analogues, therefore, cross reaction may still exist.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseMouse Rapgef2 ELISA Kit allows for the in vitro quantitative determination of Rapgef2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of RAPGEF2 in Mouse serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse RAPGEF2. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse RAPGEF2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse RAPGEF2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse RAPGEF2. You can calculate the concentration of Mouse RAPGEF2 in the samples by comparing the OD of the samples to the standard curve.
  • Mouse Rap guanine nucleotide exchange factor 2 Protein information
  • Uniprot ID RPGF2_MOUSE
  • Uniprot AC Q8CHG7; E9QMD0;
  • UniGene Mm.31220; Mm.462396;
  • GeneID 76089
  • Mouse Rap guanine nucleotide exchange factor 2 Protein SEQUENCE
  • SEQUENCE 1496 AA; 166415 MW; C2FDE48E5E21A86F CRC64;

    MKPLAAPANH GVLGQQEKQS LPADFTKLHL TDSLHPQVTH VSSSHSGCSI

    TSDSGSSSLS DIYQATESEA GDMDLSGLPE TAVDSEDDDD EEDIERASDP

    LMSRDIVRDC LEKDPIDRTD DDIEQLLEFM HQLPAFANMT MSVRRELCAV

    MVFAVVERAG TIVLNDGEEL DSWSVILNGS VEVTYPDGKA EILCMGNSFG

    VSPTMDKEYM KGVMRTKVDD CQFVCIAQQD YCRILNQVEK NMQKVEEEGE

    IVMVKEHREL DRTGTRKGHI VIKGTSERLT MHLVEEHSVV DPTFIEDFLL

    TYRTFLSSPM EVGKKLLEWF NDPSLRDKVT RVVLLWVNNH FNDFEGDPAM

    TRFLEEFENN LEREKMGGHL RLLNIACAAK AKRRLMTLTK PSREAPLPFI

    LLGGSEKGFG IFVDSVDSCS KATEAGLKRG DQILEVNGQN FENIQLSKAM

    EILRNNTHLS ITVKTNLFVF KELLTRLSEE KRNGAPHLPK IGDIKKASRY

    SIPDLAVDVE QVIGLEKVNK KSKANTVGGR NKLKKILDKT RISILPQKPY

    NDIGIGQSQD DSIVGLRQTK HIPAALPVSG TLSSSNPDLL QSHHRILDFS

    TTPDLPDQVL RVFKADQQSR YIMISKDTTA KEVVIQAIRE FAVTATPEQY

    SLCEVSVTPE GVIKQRRLPD QLSKLADRIQ LSGRYYLKNN METETLCSDE

    DAQELLRESQ ISLLQLSTVE VATQLSMRNF ELFRNIEPTE YIDDLFKLKS

    KTSCANLKKF EEVINQETFW VASEILRETN QLKRMKIIKH FIKIALHCRE

    CKNFNSMFAI ISGLNLAPVA RLRTTWEKLP NKYEKLFQDL QDLFDPSRNM

    AKYRNVLSGQ NLQPPVIPLF PVIKKDLTFL HEGNDSKVDG LVNFEKLRMI

    AKEIRHVGRM ASVNMDPALM FRTRKKKWRS LGSLSQGSAN ATVLDVAQTG

    GHKKRVRRSS FLNAKKLYED AQMARKVKQY LSNLELEMDE ESLQTLSLQC

    EPATSTLPKN PGDKKPVKSE TSPVAPRAGP QQKVQPQQPL AQPQPPHKVS

    QGLQVPAVSL YPSRKKVPVK DLPPFGINSP QALKKILSLS EEGSLERHRK

    QAEDTISNAS SQLSSPPTSP QSSPRKGYAL ALSGTVDNFS DSGHSEISSR

    SSIVSNSSFD SVPVSLHDER RQRHSVSIVE SNLGVGRMER RTLMEPDQYS

    LGSYAPVSES RGLYAAATVI SSPSTEELSH DQGDRASLDA ADSGRGSWTS

    CSSGSHDNIQ TIQHQRSWET LPFGHTHFDY SGDAASIWAS GGHMDQMMFS

    DHSTKYNRQN QSRESLEQAQ SRASWASSTG YWGEDSEGDT GTIKRRGGKD

    VSAEAESSSM VPVTTEEAKP VPMPAHIAVT PSTTKGLIAR KEGRYREPPP

    TPPGYVGIPI ADFPEGPCHP ARKPPDYNVA LQRSRMVARP TEAPAPGQTP

    PAAAASRPGS KPQWHKPSDA DPRLAPFQPQ GFAGAEEDED EQVSAV

  • UCSC uc008pnl.1; mouse.;



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