Human CPB1 ELISA Kit

Alternative name
Human pancreas-specific protein ELISA Kit;Human PASP ELISA Kit;Human CPB ELISA Kit;Human PCPB ELISA Kit;Human carboxypeptidase B1 ELISA Kit;Human carboxypeptidase B ELISA Kit;Human carboxypeptidase B1 (tissue) ELISA Kit;Human pancreatic carboxypeptidase B ELISA Kit;Human procarboxypeptidase B ELISA Kit;Human protaminase ELISA Kit;Human tissue carboxypeptidase B ELISA Kit;
Catalog
E006587
species
Human
GeneCPB1
Standard Curve
Other Species Mouse CPB1 ELISA Kit Bovine CPB1 ELISA Kit Canine CPB1 ELISA Kit Porcine CPB1 ELISA Kit Rat Cpb1 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of CPB1. No significant cross-reactivity or interference between CPB1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CPB1 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman CPB1 ELISA Kit allows for the in vitro quantitative determination of CPB1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyCell Biology
Product Description
specificalPrinciple of the assay: CPB1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CPB1 antibody and an CPB1-HRP conjugate. The assay sample and buffer are incubated together with CPB1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CPB1 concentration since CPB1 from samples and CPB1-HRP conjugate compete for the anti-CPB1 antibody binding site. Since the number of sites is limited, as more sites are occupied by CPB1 from the sample, fewer sites are left to bind CPB1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CPB1 concentration in each sample is interpolated from this standard curve.
Human Carboxypeptidase B Protein information
Uniprot ID CBPB1_HUMAN
Uniprot AC P15086; O60834; Q53XJ0; Q96BQ8;
UniGene Hs.477891;
GeneID 1360
KEGG hsa:1360;
Human Carboxypeptidase B Protein SEQUENCE
SEQUENCE 417 AA; 47368 MW; EBBB98B27F5D5AF9 CRC64;
MLALLVLVTV ALASAHHGGE HFEGEKVFRV NVEDENHINI IRELASTTQI
DFWKPDSVTQ IKPHSTVDFR VKAEDTVTVE NVLKQNELQY KVLISNLRNV
VEAQFDSRVR ATGHSYEKYN KWETIEAWTQ QVATENPALI SRSVIGTTFE
GRAIYLLKVG KAGQNKPAIF MDCGFHAREW ISPAFCQWFV REAVRTYGRE
IQVTELLDKL DFYVLPVLNI DGYIYTWTKS RFWRKTRSTH TGSSCIGTDP
NRNFDAGWCE IGASRNPCDE TYCGPAAESE KETKALADFI RNKLSSIKAY
LTIHSYSQMM IYPYSYAYKL GENNAELNAL AKATVKELAS LHGTKYTYGP
GATTIYPAAG GSDDWAYDQG IRYSFTFELR DTGRYGFLLP ESQIRATCEE
TFLAIKYVAS YVLEHLY
UCSC uc003ewl.4; human.;

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