Mouse Akt1 ELISA Kit

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  • Alternative name

    Mouse AKT1 kinase ELISA Kit;Mouse Protein kinase B ELISA Kit;Mouse PKB ELISA Kit;Mouse Protein kinase B alpha ELISA Kit;Mouse PKB alpha ELISA Kit;Mouse proto-oncogene c-AKT ELISA Kit;Mouse RAC-PK-alpha ELISA Kit;Mouse Thymoma viral proto-oncogene ELISA Kit;Mouse Akt ELISA Kit;Mouse Rac ELISA Kit;Mouse LTR-akt ELISA Kit;Mouse PKB/Akt ELISA Kit;Mouse PKBalpha ELISA Kit;Mouse thymoma viral proto-oncogene 1 ELISA Kit;Mouse RAC-alpha serine/threonine-protein kinase ELISA Kit;Mouse Akt1m protein ELISA Kit;Mouse protein kinase B-alpha ELISA Kit;Mouse related to A and C kinases ELISA Kit;Mouse tumor rejection antigen peptide pRLib ELISA Kit;

  • Catalog
    E065813
  • species
    Mouse
  • GeneAkt1
  • Standard CurveMouse Akt1 ELISA Kit
  • Other Species Human AKT1 ELISA KitBovine AKT1 ELISA KitRat Akt1 ELISA Kit
  • SpecificityThis assay recognizes recombinant and natural mouse RAC-PK-alpha. No significant cross-reactivity or interference was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseMouse Akt1 ELISA Kit allows for the in vitro quantitative determination of Akt1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to RAC-PK-alpha. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for RAC-PK-alpha and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain RAC-PK-alpha, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of RAC-PK-alpha in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Mouse RAC-alpha serine/threonine-protein kinase Protein information
  • Uniprot ID AKT1_MOUSE
  • Uniprot AC P31750; Q62274; Q6GSA6;
  • UniGene Mm.6645;
  • GeneID 11651
  • KEGG mmu:11651;
  • Mouse RAC-alpha serine/threonine-protein kinase Protein SEQUENCE
  • SEQUENCE 480 AA; 55707 MW; 98DF28E5EFE03730 CRC64;

    MNDVAIVKEG WLHKRGEYIK TWRPRYFLLK NDGTFIGYKE RPQDVDQRES

    PLNNFSVAQC QLMKTERPRP NTFIIRCLQW TTVIERTFHV ETPEEREEWA

    TAIQTVADGL KRQEEETMDF RSGSPSDNSG AEEMEVSLAK PKHRVTMNEF

    EYLKLLGKGT FGKVILVKEK ATGRYYAMKI LKKEVIVAKD EVAHTLTENR

    VLQNSRHPFL TALKYSFQTH DRLCFVMEYA NGGELFFHLS RERVFSEDRA

    RFYGAEIVSA LDYLHSEKNV VYRDLKLENL MLDKDGHIKI TDFGLCKEGI

    KDGATMKTFC GTPEYLAPEV LEDNDYGRAV DWWGLGVVMY EMMCGRLPFY

    NQDHEKLFEL ILMEEIRFPR TLGPEAKSLL SGLLKKDPTQ RLGGGSEDAK

    EIMQHRFFAN IVWQDVYEKK LSPPFKPQVT SETDTRYFDE EFTAQMITIT

    PPDQDDSMEC VDSERRPHFP QFSYSASGTA

  • UCSC uc007pex.2; mouse.;


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