Human CPA3 ELISA Kit

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  • Alternative name

    Human MC-CPA ELISA Kit;Human carboxypeptidase A3 ELISA Kit;Human mast cell carboxypeptidase A ELISA Kit;Human carboxypeptidase A3 (mast cell) ELISA Kit;Human tissue carboxypeptidase A ELISA Kit;

  • Catalog
    E006575
  • species
    Human
  • GeneCPA3
  • Other Species Mouse Cpa3 ELISA KitRat Cpa3 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of human mast cell carboxypeptidase. No significant cross-reactivity or interference between human mast cell carboxypeptidase and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of human mast cell carboxypeptidase is typically less than 0.078 ng/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It
  • Intended UseHuman CPA3 ELISA Kit allows for the in vitro quantitative determination of CPA3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for mast cell carboxypeptidase has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any mast cell carboxypeptidase present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for mast cell carboxypeptidase is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of mast cell carboxypeptidase bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Human Mast cell carboxypeptidase A Protein information
  • Uniprot ID CBPA3_HUMAN
  • Uniprot AC P15088; Q96E94;
  • UniGene Hs.646;
  • GeneID 1359
  • KEGG hsa:1359;
  • Human Mast cell carboxypeptidase A Protein SEQUENCE
  • SEQUENCE 417 AA; 48670 MW; ACB9038758117F9A CRC64;

    MRLILPVGLI ATTLAIAPVR FDREKVFRVK PQDEKQADII KDLAKTNELD

    FWYPGATHHV AANMMVDFRV SEKESQAIQS ALDQNKMHYE ILIHDLQEEI

    EKQFDVKEDI PGRHSYAKYN NWEKIVAWTE KMMDKYPEMV SRIKIGSTVE

    DNPLYVLKIG EKNERRKAIF TDCGIHAREW VSPAFCQWFV YQATKTYGRN

    KIMTKLLDRM NFYILPVFNV DGYIWSWTKN RMWRKNRSKN QNSKCIGTDL

    NRNFNASWNS IPNTNDPCAD NYRGSAPESE KETKAVTNFI RSHLNEIKVY

    ITFHSYSQML LFPYGYTSKL PPNHEDLAKV AKIGTDVLST RYETRYIYGP

    IESTIYPISG SSLDWAYDLG IKHTFAFELR DKGKFGFLLP ESRIKPTCRE

    TMLAVKFIAK YILKHTS

  • UCSC uc003ewm.4; human.;


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