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Mouse Rspo1 ELISA Kit

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  • Alternative name

    Mouse cysteine-rich and single thrombospondin domain-containing protein 3 ELISA Kit;Mouse cristin-3 ELISA Kit;Mouse mCristin-3 ELISA Kit;Mouse roof plate-specific spondin-1 ELISA Kit;Mouse R-spondin ELISA Kit;Mouse Rspondin ELISA Kit;Mouse R-spondin 1 ELISA Kit;Mouse R-spondin-1 ELISA Kit;Mouse R-spondin homolog ELISA Kit;Mouse thrombospondin type 1 domain containing gene Rspondin ELISA Kit;
  • Catalog
    E065723
  • species
    Mouse
  • GeneRspo1
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of RSPO1. No significant cross-reactivity or interference between RSPO1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between RSPO1 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 pg/mL.
  • Intended UseMouse Rspo1 ELISA Kit allows for the in vitro quantitative determination of Rspo1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Intended Uses: This RSPO1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse RSPO1. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||RSPO1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-RSPO1 antibody and an RSPO1-HRP conjugate. The assay sample and buffer are incubated together with RSPO1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the RSPO1 concentration since RSPO1 from samples and RSPO1-HRP conjugate compete for the anti-RSPO1 antibody binding site. Since the number of sites is limited, as more sites are occupied by RSPO1 from the sample, fewer sites are left to bind RSPO1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The RSPO1 concentration in each sample is interpolated from this standard curve.
  • Mouse R-spondin-1 Protein information
  • Uniprot ID RSPO1_MOUSE
  • Uniprot AC Q9Z132; Q3V1S3;
  • UniGene Mm.42202;
  • GeneID 192199
  • KEGG mmu:192199;
  • Mouse R-spondin-1 Protein SEQUENCE

  • SEQUENCE 265 AA; 29332 MW; FFEB8964743F5963 CRC64;

    MRLGLCVVAL VLSWTHIAVG SRGIKGKRQR RISAEGSQAC AKGCELCSEV

    NGCLKCSPKL FILLERNDIR QVGVCLPSCP PGYFDARNPD MNKCIKCKIE

    HCEACFSHNF CTKCQEALYL HKGRCYPACP EGSTAANSTM ECGSPAQCEM

    SEWSPWGPCS KKRKLCGFRK GSEERTRRVL HAPGGDHTTC SDTKETRKCT

    VRRTPCPEGQ KRRKGGQGRR ENANRHPARK NSKEPRSNSR RHKGQQQPQP

    GTTGPLTSVG PTWAQ




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