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Alternative name
Pyruvate kinase PKM ELISA KIT; Cytosolic thyroid hormone-binding protein ELISA KIT; CTHBP ELISA KIT; Opa-interacting protein 3 ELISA KIT; OIP-3 ELISA KIT; Pyruvate kinase 2/3 ELISA KIT; Pyruvate kinase muscle isozyme ELISA KIT; Thyroid hormone-binding protein 1 ELISA KIT; THBP1 ELISA KIT; Tumor M2-PK ELISA KIT; p58PKM ELISA KIT; OIP3 ELISA KIT; PK2 ELISA KIT; PK3 ELISA KIT; PKM2 ELISA KIT; CTHBP ELISA KIT; OIP-3 ELISA KIT; THBP1 ELISA KIT
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Catalog
E065701
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species
Mouse
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GenePKM2
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Pyruvate Kinase, Muscle (PKM2).
No significant cross-reactivity or interference between Pyruvate Kinase, Muscle (PKM2) and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityTypically less than 0.127ng/mL.
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Intended UseMouse PKM2 ELISA Kit allows for the in vitro quantitative determination of PKM2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Pyruvate Kinase, Muscle (PKM2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Pyruvate Kinase, Muscle (PKM2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Pyruvate Kinase, Muscle (PKM2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Pyruvate Kinase, Muscle (PKM2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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