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Alternative name
Nectin-1 ELISA KIT; Herpes virus entry mediator C ELISA KIT; Herpesvirus entry mediator C ELISA KIT; HveC ELISA KIT; Herpesvirus Ig-like receptor ELISA KIT; HIgR ELISA KIT; Poliovirus receptor-related protein 1 ELISA KIT; CD_antigen: CD111PVRL1 ELISA KIT; HVEC ELISA KIT; PRR1 ELISA KIT; Herpesvirus entry mediator C ELISA KIT; HveC ELISA KIT; HIgR ELISA KIT
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Catalog
E065612
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species
Mouse
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GenePVRL1
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse PVRL1. No significant cross-reactivity or interference between Mouse PVRL1 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse PVRL1 and all the analogues, therefore, cross reaction may still exist.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Intended UseMouse PVRL1 ELISA Kit allows for the in vitro quantitative determination of PVRL1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalDescription: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PVRL1 in Mouse serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse PVRL1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse PVRL1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PVRL1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse PVRL1. You can calculate the concentration of Mouse PVRL1 in the samples by comparing the OD of the samples to the standard curve.
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