Human CES1 ELISA Kit

Alternative name
Human Acyl-coenzyme A:cholesterol acyltransferase ELISA Kit;Human ACAT ELISA Kit;Human brain carboxylesterase hBr1 ELISA Kit;Human carboxylesterase 1 ELISA Kit;Human CE-1 ELISA Kit;Human hCE-1 ELISA Kit;Human cocaine carboxylesterase ELISA Kit;Human egasyn ELISA Kit;Human HMSE ELISA Kit;Human methylumbelliferyl-acetate deacetylase 1 ELISA Kit;Human monocyte/macrophage serine esterase ELISA Kit;Human retinyl ester hydrolase ELISA Kit;Human REH ELISA Kit;Human serine esterase 1 ELISA Kit;Human triacylglycerol hydrolase ELISA Kit;Human TGH ELISA Kit;Human CES2 ELISA Kit;Human SES1 ELISA Kit;Human CEH ELISA Kit;Human HMSE1 ELISA Kit;Human PCE-1 ELISA Kit;Human liver carboxylesterase 1 ELISA Kit;Human acyl coenzyme A:cholesterol acyltransferase ELISA Kit;Human carboxylesterase 1 (monocyte/macrophage serine esterase 1) ELISA Kit;Human carboxylesterase 2 (liver) ELISA Kit;Human cholesteryl ester hydrolase ELISA Kit;Human human monocyte/macrophage serine esterase 1 ELISA Kit;
Catalog
E006557
species
Human
GeneCES1
Standard Curve
Other Species Mouse Ces1 ELISA Kit Monkey CES1 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of Carboxylesterase 1 (CES1). No significant cross-reactivity or interference between Carboxylesterase 1 (CES1) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.055ng/mL.
Intended UseHuman CES1 ELISA Kit allows for the in vitro quantitative determination of CES1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carboxylesterase 1 (CES1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Carboxylesterase 1 (CES1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Carboxylesterase 1 (CES1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Carboxylesterase 1 (CES1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Liver carboxylesterase 1 Protein information
Uniprot ID EST1_HUMAN
Uniprot AC Q9UDG8; Q9UK77; Q9ULY2;
UniGene Hs.558865;
GeneID 1066
KEGG hsa:1066;
Human Liver carboxylesterase 1 Protein SEQUENCE
SEQUENCE 567 AA; 62521 MW; D3A00BDCDC7E5DFF CRC64;
MWLRAFILAT LSASAAWGHP SSPPVVDTVH GKVLGKFVSL EGFAQPVAIF
LGIPFAKPPL GPLRFTPPQP AEPWSFVKNA TSYPPMCTQD PKAGQLLSEL
FTNRKENIPL KLSEDCLYLN IYTPADLTKK NRLPVMVWIH GGGLMVGAAS
TYDGLALAAH ENVVVVTIQY RLGIWGFFST GDEHSRGNWG HLDQVAALRW
VQDNIASFGG NPGSVTIFGE SAGGESVSVL VLSPLAKNLF HRAISESGVA
LTSVLVKKGD VKPLAEQIAI TAGCKTTTSA VMVHCLRQKT EEELLETTLK
MKFLSLDLQG DPRESQPLLG TVIDGMLLLK TPEELQAERN FHTVPYMVGI
NKQEFGWLIP MQLMSYPLSE GQLDQKTAMS LLWKSYPLVC IAKELIPEAT
EKYLGGTDDT VKKKDLFLDL IADVMFGVPS VIVARNHRDA GAPTYMYEFQ
YRPSFSSDMK PKTVIGDHGD ELFSVFGAPF LKEGASEEEI RLSKMVMKFW
ANFARNGNPN GEGLPHWPEY NQKEGYLQIG ANTQAAQKLK DKEVAFWTNL
FAKKAVEKPP QTEHIEL
UCSC uc002eil.4; human. [P23141-1];

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Human CES1 ELISA Kit